|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R3 to EC50 for wild type Pestalotiopsis microspora isolate CA-8
|
Pestalotiopsis microspora
|
1.05
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R2 to EC50 for wild type Pestalotiopsis microspora isolate CA-8
|
Pestalotiopsis microspora
|
0.95
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R1 to EC50 for wild type Pestalotiopsis microspora isolate CA-8
|
Pestalotiopsis microspora
|
0.97
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate LA1R2 to EC50 for wild type Pestalotiopsis microspora isolate LA-1
|
Pestalotiopsis microspora
|
1.02
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate LA1R1 to EC50 for wild type Pestalotiopsis microspora isolate LA-1
|
Pestalotiopsis microspora
|
0.98
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R3 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
19.0
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R2 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
17.7
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R1 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
18.1
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against wild type Pestalotiopsis microspora isolate CA-8 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
18.6
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate LA1R2 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
20.6
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate LA1R1 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
19.7
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against wild type Pestalotiopsis microspora isolate LA-1 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
20.1
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against Pestalotiopsis microspora infected in Chinese hickory orchards assessed as conidium germination inhibition at 25 degC after 24 hr
|
Pestalotiopsis microspora
|
23.92
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against Pestalotiopsis microspora infected Chinese hickory orchards assessed as mycelial growth inhibition at 25 degC after 5 days
|
Pestalotiopsis microspora
|
166.93
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Antifungal activity against mepronil-resistant Rhizoctonia solani JSR3 after 2 days
|
Rhizoctonia solani
|
2.08
ug.mL-1
|
|
Journal : Crop Protection
Year : 2009
Volume : 28
Issue : 5
First Page : 381
Last Page : 386
|
Antifungal activity against mepronil-resistant Rhizoctonia solani JSR2 after 2 days
|
Rhizoctonia solani
|
2.13
ug.mL-1
|
|
Journal : Crop Protection
Year : 2009
Volume : 28
Issue : 5
First Page : 381
Last Page : 386
|
Antifungal activity against mepronil-resistant Rhizoctonia solani JSR1 after 2 days
|
Rhizoctonia solani
|
2.26
ug.mL-1
|
|
Journal : Crop Protection
Year : 2009
Volume : 28
Issue : 5
First Page : 381
Last Page : 386
|
Antifungal activity against mepronil-sensitive wild type Rhizoctonia solani JS7 after 2 days
|
Rhizoctonia solani
|
2.15
ug.mL-1
|
|
Journal : Crop Protection
Year : 2009
Volume : 28
Issue : 5
First Page : 381
Last Page : 386
|
Antifungal activity against mepronil-resistant Rhizoctonia solani ZJR2 after 2 days
|
Rhizoctonia solani
|
2.15
ug.mL-1
|
|
Journal : Crop Protection
Year : 2009
Volume : 28
Issue : 5
First Page : 381
Last Page : 386
|
Antifungal activity against mepronil-resistant Rhizoctonia solani ZJR3 after 2 days
|
Rhizoctonia solani
|
1.93
ug.mL-1
|
|
Journal : Crop Protection
Year : 2009
Volume : 28
Issue : 5
First Page : 381
Last Page : 386
|
Antifungal activity against mepronil-resistant Rhizoctonia solani ZJR1 after 2 days
|
Rhizoctonia solani
|
1.87
ug.mL-1
|
|
Journal : Crop Protection
Year : 2009
Volume : 28
Issue : 5
First Page : 381
Last Page : 386
|
Antifungal activity against mepronil-sensitive wild type Rhizoctonia solani ZJ-1 after 2 days
|
Rhizoctonia solani
|
1.98
ug.mL-1
|
|
Journal : Crop Protection
Year : 2009
Volume : 28
Issue : 5
First Page : 381
Last Page : 386
|
Antifungal activity against Sclerotinia sclerotiorum in oilseed rape field assessed as disease control at 250/ha measured 1 week before harvest
|
Sclerotinia sclerotiorum
|
75.1
%
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against Sclerotinia sclerotiorum in oilseed rape field assessed as disease control at 125/ha measured 1 week before harvest
|
Sclerotinia sclerotiorum
|
65.2
%
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against Sclerotinia sclerotiorum in oilseed rape field assessed as disease control at 187.5/ha measured 1 week before harvest
|
Sclerotinia sclerotiorum
|
69.0
%
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against Sclerotinia sclerotiorum in oilseed rape field assessed as disease control at 80/ha measured 1 week before harvest
|
Sclerotinia sclerotiorum
|
49.7
%
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against Sclerotinia sclerotiorum in synthetic medium assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.12
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against Sclerotinia sclerotiorum in czapek medium assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.13
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against Sclerotinia sclerotiorum in PDA plates assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.17
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-resistant Sclerotinia sclerotiorum CZ57-2 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.183
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-resistant Sclerotinia sclerotiorum CZ57-3 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.163
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-resistant Sclerotinia sclerotiorum ZJ288-3 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.262
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-resistant Sclerotinia sclerotiorum CZ57-1 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.115
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-resistant Sclerotinia sclerotiorum ZJ288-2 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.126
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-sensitive Sclerotinia sclerotiorum SZ48 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.168
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-resistant Sclerotinia sclerotiorum ZJ288-1 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.195
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-sensitive Sclerotinia sclerotiorum ZJ47 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.188
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-sensitive Sclerotinia sclerotiorum TZ83 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.19
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-sensitive Sclerotinia sclerotiorum YC35 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.195
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-sensitive Sclerotinia sclerotiorum ZJ44 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.223
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-sensitive Sclerotinia sclerotiorum CZ57 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.186
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim-resistant and dimethachlon-sensitive Sclerotinia sclerotiorum ZJ288 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.18
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim and dimethachlon-sensitive Sclerotinia sclerotiorum SZ47 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.181
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim and dimethachlon-sensitive Sclerotinia sclerotiorum SZ62 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.17
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim and dimethachlon-sensitive Sclerotinia sclerotiorum YZ116 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.195
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim and dimethachlon-sensitive Sclerotinia sclerotiorum YZ114 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.195
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Antifungal activity against carbendazim and dimethachlon-sensitive Sclerotinia sclerotiorum YZ112 assessed as inhibition of mycelial growth after 2 days
|
Sclerotinia sclerotiorum
|
0.211
ug.mL-1
|
|
Journal : Crop Protection
Title : Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China
Year : 2009
Volume : 28
Issue : 10
First Page : 882
Last Page : 886
Authors : Wang Jian-Xin, Ma Hui-Xia, Chen Yu, Zhu Xiao-Fen, Yu Wen-Yuan, Tang Zheng-He, Chen Chang-Jun, Zhou Ming-Guo.
Abstract : Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
|
Fungicidal activity against Monilinia laxa isolate MLX1 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
60.03
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLP4 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
0.0
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLK12 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
45.05
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLK1 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
62.05
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLC2 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
0.0
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLX5 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
0.0
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLP2 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
66.82
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLBO5 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
0.0
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLBO2 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
36.67
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLA1 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
42.73
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLG1 assessed as mycelial growth at 0.5 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
0.0
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Antifungal activity against Botryotinia fuckeliana in Cucumber seedlings assessed as reduction of gray mold diseases compound spraying was performed 3 hr prior to inoculation by preventive effect assay
|
Botryotinia fuckeliana
|
0.8
ug.mL-1
|
|
Journal : J Pesticide Sci
Year : 2010
Volume : 35
Issue : 1
First Page : 10
Last Page : 14
|
Protective antifungal activity against Botryotinia fuckeliana inoculated in fungicide-applied strawberry fruit assessed as disease severity measuring lesion diameter at 300 ug AI/ml applied 96 hr prior-inoculation
|
Botryotinia fuckeliana
|
3.4
mm
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae MDR to EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR1 by germ tube elongation assay
|
Oculimacula yallundae
|
4.5
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae MDR to EC50 for wild type Oculimacula yallundae TriS by germ tube elongation assay
|
Oculimacula yallundae
|
5.0
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR2 to EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR1 by germ tube elongation assay
|
Oculimacula yallundae
|
1.2
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR2 to EC50 for wild type Oculimacula yallundae TriS by germ tube elongation assay
|
Oculimacula yallundae
|
1.3
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR1 to EC50 for wild type Oculimacula yallundae TriS by germ tube elongation assay
|
Oculimacula yallundae
|
1.1
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae MDR to EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR1 by mycelial growth inhibition assay
|
Oculimacula yallundae
|
10.0
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Selectivity index, ratio of EC50 for Oculimacula acuformis to EC50 for Oculimacula yallundae in presence of 5 g sodium succinate by mycelial growth inhibition assay
|
None
|
0.06
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Fungicidal activity against Oculimacula yallundae assessed as inhibition of mycelial growth in presence of 5 g sodium succinate incubated at 19 degC in dark for 4 weeks
|
Oculimacula yallundae
|
5.0
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR1 to EC50 for wild type Oculimacula yallundae TriS by mycelial growth inhibition assay
|
Oculimacula yallundae
|
0.8
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Fungicidal activity against Oculimacula acuformis assessed as inhibition of mycelial growth in presence of 5 g sodium succinate incubated at 19 degC in dark for 4 weeks
|
Oculimacula acuformis
|
0.3
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Selectivity index, ratio of EC50 for Oculimacula acuformis to EC50 for Oculimacula yallundae in presence of 10 g glucose by mycelial growth inhibition assay
|
None
|
0.08
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Fungicidal activity against Oculimacula yallundae assessed as inhibition of mycelial growth in presence of 10 g glucose incubated at 19 degC in dark for 4 weeks
|
Oculimacula yallundae
|
0.4
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Fungicidal activity against Oculimacula acuformis assessed as inhibition of mycelial growth in presence of 10 g glucose incubated at 19 degC in dark for 4 weeks
|
Oculimacula acuformis
|
0.3
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Selectivity index, ratio of EC50 for Oculimacula acuformis to EC50 for Oculimacula yallundae by germ tube elongation inhibition assay
|
None
|
0.8
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Fungicidal activity against Oculimacula yallundae assessed as inhibition of germ tube elongation incubated at 19 degC in dark for 48 hr
|
Oculimacula yallundae
|
0.03
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Fungicidal activity against Oculimacula acuformis assessed as inhibition of germ tube elongation incubated at 19 degC in dark for 48 hr
|
Oculimacula acuformis
|
0.025
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR2 to EC50 for wild type Oculimacula yallundae TriS by mycelial growth inhibition assay
|
Oculimacula yallundae
|
1.0
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR2 to EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae TriR1 by mycelial growth inhibition assay
|
Oculimacula yallundae
|
1.3
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Resistance index, ratio of EC50 for sterol 14alpha-demethylation inhibitor-resistant Oculimacula yallundae MDR to EC50 for wild type Oculimacula yallundae TriS by mycelial growth inhibition assay
|
Oculimacula yallundae
|
7.5
|
|
Journal : Pest Manag Sci
Title : Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae.
Year : 2013
Volume : 69
Issue : 1
First Page : 15
Last Page : 26
Authors : Leroux P, Gredt M, Remuson F, Micoud A, Walker AS.
Abstract : Eyespot, caused by Oculimacula acuformis and Oculimacula yallundae, is the major foot disease of winter wheat in several European countries, including France. It can be controlled by chemical treatment between tillering and the second node stage. The fungicides used include antimicrotubule toxicants (benzimidazoles), inhibitors of sterol 14α-demethylation (DMIs) or of succinate dehydrogenase (SDHIs), the anilinopyrimidines cyprodinil and the benzophenone metrafenone. Since the early 1980s, a long-term survey has been set up in France to monitor changes in the sensitivity of eyespot populations to fungicides. Resistance to benzimidazoles has become generalised since the early 1990s, in spite of the withdrawal of this class of fungicides. In the DMI group, resistance to triazoles is generalised, whereas no resistance to the triazolinethione prothioconazole has yet developed. Resistance to the imidazole prochloraz evolved successively in O. acuformis and O. yallundae and is now well established. Specific resistance to cyprodinil has also been detected, but its frequency has generally remained low. Finally, since the early 2000s, a few strains of O. yallundae displaying multidrug resistance (MDR) have been detected. These strains display low levels of resistance to prothioconazole and SDHIs, such as boscalid. Knowledge of the spatiotemporal distribution in France of O. acuformis and O. yallundae field strains resistant to fungicides allows resistance management strategies for eyespot fungi in winter wheat to be proposed.
|
Antifungal activity against Fusarium oxysporum assessed as inhibition of mycelium growth at 100 ug/ml
|
Fusarium oxysporum
|
4.3
%
|
|
Journal : Molecules
Year : 2012
Volume : 17
Issue : 12
First Page : 14205
Last Page : 14218
|
Antifungal activity against Cytospora mandshurica assessed as inhibition of mycelium growth at 100 ug/ml
|
Cytospora
|
3.4
%
|
|
Journal : Molecules
Year : 2012
Volume : 17
Issue : 12
First Page : 14205
Last Page : 14218
|
Antifungal activity against Fusarium graminearum assessed as inhibition of mycelium growth at 100 ug/ml
|
Fusarium graminearum
|
35.9
%
|
|
Journal : Molecules
Year : 2012
Volume : 17
Issue : 12
First Page : 14205
Last Page : 14218
|
Fungicidal activity against Zymoseptoria tritici isolate TAG74-3 with 120 bp insertion expressing CYP51 variant with combination of L50S, S188N, I381V, deltaY459/G460 and N513K mutations assessed as fungal growth inhibition
|
Zymoseptoria tritici
|
0.719
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
Year : 2012
Volume : 68
Issue : 7
First Page : 1034
Last Page : 1040
Authors : Cools HJ, Bayon C, Atkins S, Lucas JA, Fraaije BA.
Abstract : The recent evolution towards resistance to azole fungicides in European populations of the wheat pathogen Mycosphaerella graminicola has been caused by the progressive accumulation of mutations in MgCYP51 gene, encoding the azole target sterol 14α-demethylase. Particular combinations of mutations have been shown specifically to affect the interaction of the MgCYP51 protein with different members of the azole class. Although additional mechanisms, including increased MgCYP51 expression and enhanced active efflux, have been proposed, the genetic changes underlying these mechanisms are unknown.Analysis of the azole sensitivities of recent M. graminicola isolates identified a novel phenotype, seemingly independent of changes in MgCYP51 coding sequence. Characterised by a 7-16-fold reduction in in vitro sensitivity to all azoles tested and by growth on seedlings at higher doses of azoles in glasshouse tests compared with isolates carrying the same MgCYP51 variant (L50S, S188N, I381V, ΔY459/G460, N513K), isolates with this phenotype constitutively overexpress MgCYP51 by between 10- and 40-fold compared with the wild type. Analysis of sequences upstream of the predicted MgCYP51 translation start codon identified a novel 120 bp indel, considered to be an insertion, in isolates overexpressing MgCYP51.The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.
|
Fungicidal activity against Zymoseptoria tritici isolate OP7 with 120 bp insertion expressing CYP51 variant with combination of L50S, S188N, I381V, deltaY459/G460 and N513K mutations assessed as fungal growth inhibition
|
Zymoseptoria tritici
|
0.808
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
Year : 2012
Volume : 68
Issue : 7
First Page : 1034
Last Page : 1040
Authors : Cools HJ, Bayon C, Atkins S, Lucas JA, Fraaije BA.
Abstract : The recent evolution towards resistance to azole fungicides in European populations of the wheat pathogen Mycosphaerella graminicola has been caused by the progressive accumulation of mutations in MgCYP51 gene, encoding the azole target sterol 14α-demethylase. Particular combinations of mutations have been shown specifically to affect the interaction of the MgCYP51 protein with different members of the azole class. Although additional mechanisms, including increased MgCYP51 expression and enhanced active efflux, have been proposed, the genetic changes underlying these mechanisms are unknown.Analysis of the azole sensitivities of recent M. graminicola isolates identified a novel phenotype, seemingly independent of changes in MgCYP51 coding sequence. Characterised by a 7-16-fold reduction in in vitro sensitivity to all azoles tested and by growth on seedlings at higher doses of azoles in glasshouse tests compared with isolates carrying the same MgCYP51 variant (L50S, S188N, I381V, ΔY459/G460, N513K), isolates with this phenotype constitutively overexpress MgCYP51 by between 10- and 40-fold compared with the wild type. Analysis of sequences upstream of the predicted MgCYP51 translation start codon identified a novel 120 bp indel, considered to be an insertion, in isolates overexpressing MgCYP51.The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.
|
Fungicidal activity against Zymoseptoria tritici isolate TAG1-18 with 120 bp insertion expressing CYP51 variant with combination of L50S, S188N, I381V, deltaY459/G460 and N513K mutations assessed as fungal growth inhibition
|
Zymoseptoria tritici
|
0.976
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
Year : 2012
Volume : 68
Issue : 7
First Page : 1034
Last Page : 1040
Authors : Cools HJ, Bayon C, Atkins S, Lucas JA, Fraaije BA.
Abstract : The recent evolution towards resistance to azole fungicides in European populations of the wheat pathogen Mycosphaerella graminicola has been caused by the progressive accumulation of mutations in MgCYP51 gene, encoding the azole target sterol 14α-demethylase. Particular combinations of mutations have been shown specifically to affect the interaction of the MgCYP51 protein with different members of the azole class. Although additional mechanisms, including increased MgCYP51 expression and enhanced active efflux, have been proposed, the genetic changes underlying these mechanisms are unknown.Analysis of the azole sensitivities of recent M. graminicola isolates identified a novel phenotype, seemingly independent of changes in MgCYP51 coding sequence. Characterised by a 7-16-fold reduction in in vitro sensitivity to all azoles tested and by growth on seedlings at higher doses of azoles in glasshouse tests compared with isolates carrying the same MgCYP51 variant (L50S, S188N, I381V, ΔY459/G460, N513K), isolates with this phenotype constitutively overexpress MgCYP51 by between 10- and 40-fold compared with the wild type. Analysis of sequences upstream of the predicted MgCYP51 translation start codon identified a novel 120 bp indel, considered to be an insertion, in isolates overexpressing MgCYP51.The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.
|
Fungicidal activity against Zymoseptoria tritici isolate V18 with 120 bp insertion expressing CYP51 variant with combination of L50S, S188N, I381V, deltaY459/G460 and N513K mutations assessed as fungal growth inhibition
|
Zymoseptoria tritici
|
None
|
|
Journal : Pest Manag Sci
Title : Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
Year : 2012
Volume : 68
Issue : 7
First Page : 1034
Last Page : 1040
Authors : Cools HJ, Bayon C, Atkins S, Lucas JA, Fraaije BA.
Abstract : The recent evolution towards resistance to azole fungicides in European populations of the wheat pathogen Mycosphaerella graminicola has been caused by the progressive accumulation of mutations in MgCYP51 gene, encoding the azole target sterol 14α-demethylase. Particular combinations of mutations have been shown specifically to affect the interaction of the MgCYP51 protein with different members of the azole class. Although additional mechanisms, including increased MgCYP51 expression and enhanced active efflux, have been proposed, the genetic changes underlying these mechanisms are unknown.Analysis of the azole sensitivities of recent M. graminicola isolates identified a novel phenotype, seemingly independent of changes in MgCYP51 coding sequence. Characterised by a 7-16-fold reduction in in vitro sensitivity to all azoles tested and by growth on seedlings at higher doses of azoles in glasshouse tests compared with isolates carrying the same MgCYP51 variant (L50S, S188N, I381V, ΔY459/G460, N513K), isolates with this phenotype constitutively overexpress MgCYP51 by between 10- and 40-fold compared with the wild type. Analysis of sequences upstream of the predicted MgCYP51 translation start codon identified a novel 120 bp indel, considered to be an insertion, in isolates overexpressing MgCYP51.The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.
|
Fungicidal activity against Zymoseptoria tritici isolate OP6 without 120 bp insertion expressing CYP51 variant with combination of L50S, S188N, I381V, deltaY459/G460 and N513K mutations assessed as fungal growth inhibition
|
Zymoseptoria tritici
|
0.841
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
Year : 2012
Volume : 68
Issue : 7
First Page : 1034
Last Page : 1040
Authors : Cools HJ, Bayon C, Atkins S, Lucas JA, Fraaije BA.
Abstract : The recent evolution towards resistance to azole fungicides in European populations of the wheat pathogen Mycosphaerella graminicola has been caused by the progressive accumulation of mutations in MgCYP51 gene, encoding the azole target sterol 14α-demethylase. Particular combinations of mutations have been shown specifically to affect the interaction of the MgCYP51 protein with different members of the azole class. Although additional mechanisms, including increased MgCYP51 expression and enhanced active efflux, have been proposed, the genetic changes underlying these mechanisms are unknown.Analysis of the azole sensitivities of recent M. graminicola isolates identified a novel phenotype, seemingly independent of changes in MgCYP51 coding sequence. Characterised by a 7-16-fold reduction in in vitro sensitivity to all azoles tested and by growth on seedlings at higher doses of azoles in glasshouse tests compared with isolates carrying the same MgCYP51 variant (L50S, S188N, I381V, ΔY459/G460, N513K), isolates with this phenotype constitutively overexpress MgCYP51 by between 10- and 40-fold compared with the wild type. Analysis of sequences upstream of the predicted MgCYP51 translation start codon identified a novel 120 bp indel, considered to be an insertion, in isolates overexpressing MgCYP51.The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.
|
Fungicidal activity against Zymoseptoria tritici isolate R10-13 without 120 bp insertion expressing CYP51 variant with combination of L50S, S188N, I381V, deltaY459/G460 and N513K mutations assessed as fungal growth inhibition
|
Zymoseptoria tritici
|
None
|
|
Journal : Pest Manag Sci
Title : Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
Year : 2012
Volume : 68
Issue : 7
First Page : 1034
Last Page : 1040
Authors : Cools HJ, Bayon C, Atkins S, Lucas JA, Fraaije BA.
Abstract : The recent evolution towards resistance to azole fungicides in European populations of the wheat pathogen Mycosphaerella graminicola has been caused by the progressive accumulation of mutations in MgCYP51 gene, encoding the azole target sterol 14α-demethylase. Particular combinations of mutations have been shown specifically to affect the interaction of the MgCYP51 protein with different members of the azole class. Although additional mechanisms, including increased MgCYP51 expression and enhanced active efflux, have been proposed, the genetic changes underlying these mechanisms are unknown.Analysis of the azole sensitivities of recent M. graminicola isolates identified a novel phenotype, seemingly independent of changes in MgCYP51 coding sequence. Characterised by a 7-16-fold reduction in in vitro sensitivity to all azoles tested and by growth on seedlings at higher doses of azoles in glasshouse tests compared with isolates carrying the same MgCYP51 variant (L50S, S188N, I381V, ΔY459/G460, N513K), isolates with this phenotype constitutively overexpress MgCYP51 by between 10- and 40-fold compared with the wild type. Analysis of sequences upstream of the predicted MgCYP51 translation start codon identified a novel 120 bp indel, considered to be an insertion, in isolates overexpressing MgCYP51.The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.
|
Fungicidal activity against Zymoseptoria tritici isolate R03-54 without 120 bp insertion expressing CYP51 variant with combination of L50S, S188N, I381V, deltaY459/G460 and N513K mutations assessed as fungal growth inhibition
|
Zymoseptoria tritici
|
0.766
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
Year : 2012
Volume : 68
Issue : 7
First Page : 1034
Last Page : 1040
Authors : Cools HJ, Bayon C, Atkins S, Lucas JA, Fraaije BA.
Abstract : The recent evolution towards resistance to azole fungicides in European populations of the wheat pathogen Mycosphaerella graminicola has been caused by the progressive accumulation of mutations in MgCYP51 gene, encoding the azole target sterol 14α-demethylase. Particular combinations of mutations have been shown specifically to affect the interaction of the MgCYP51 protein with different members of the azole class. Although additional mechanisms, including increased MgCYP51 expression and enhanced active efflux, have been proposed, the genetic changes underlying these mechanisms are unknown.Analysis of the azole sensitivities of recent M. graminicola isolates identified a novel phenotype, seemingly independent of changes in MgCYP51 coding sequence. Characterised by a 7-16-fold reduction in in vitro sensitivity to all azoles tested and by growth on seedlings at higher doses of azoles in glasshouse tests compared with isolates carrying the same MgCYP51 variant (L50S, S188N, I381V, ΔY459/G460, N513K), isolates with this phenotype constitutively overexpress MgCYP51 by between 10- and 40-fold compared with the wild type. Analysis of sequences upstream of the predicted MgCYP51 translation start codon identified a novel 120 bp indel, considered to be an insertion, in isolates overexpressing MgCYP51.The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.
|
Fungicidal activity against Zymoseptoria tritici isolate R03-29 without 120 bp insertion expressing CYP51 variant with combination of L50S, S188N, I381V, deltaY459/G460 and N513K mutations assessed as fungal growth inhibition
|
Zymoseptoria tritici
|
0.21
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
Year : 2012
Volume : 68
Issue : 7
First Page : 1034
Last Page : 1040
Authors : Cools HJ, Bayon C, Atkins S, Lucas JA, Fraaije BA.
Abstract : The recent evolution towards resistance to azole fungicides in European populations of the wheat pathogen Mycosphaerella graminicola has been caused by the progressive accumulation of mutations in MgCYP51 gene, encoding the azole target sterol 14α-demethylase. Particular combinations of mutations have been shown specifically to affect the interaction of the MgCYP51 protein with different members of the azole class. Although additional mechanisms, including increased MgCYP51 expression and enhanced active efflux, have been proposed, the genetic changes underlying these mechanisms are unknown.Analysis of the azole sensitivities of recent M. graminicola isolates identified a novel phenotype, seemingly independent of changes in MgCYP51 coding sequence. Characterised by a 7-16-fold reduction in in vitro sensitivity to all azoles tested and by growth on seedlings at higher doses of azoles in glasshouse tests compared with isolates carrying the same MgCYP51 variant (L50S, S188N, I381V, ΔY459/G460, N513K), isolates with this phenotype constitutively overexpress MgCYP51 by between 10- and 40-fold compared with the wild type. Analysis of sequences upstream of the predicted MgCYP51 translation start codon identified a novel 120 bp indel, considered to be an insertion, in isolates overexpressing MgCYP51.The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.
|
Fungicidal activity against Zymoseptoria tritici isolate IPO323 without 120 bp insertion expressing wild type CYP51 assessed as fungal growth inhibition
|
Zymoseptoria tritici
|
0.554
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
Year : 2012
Volume : 68
Issue : 7
First Page : 1034
Last Page : 1040
Authors : Cools HJ, Bayon C, Atkins S, Lucas JA, Fraaije BA.
Abstract : The recent evolution towards resistance to azole fungicides in European populations of the wheat pathogen Mycosphaerella graminicola has been caused by the progressive accumulation of mutations in MgCYP51 gene, encoding the azole target sterol 14α-demethylase. Particular combinations of mutations have been shown specifically to affect the interaction of the MgCYP51 protein with different members of the azole class. Although additional mechanisms, including increased MgCYP51 expression and enhanced active efflux, have been proposed, the genetic changes underlying these mechanisms are unknown.Analysis of the azole sensitivities of recent M. graminicola isolates identified a novel phenotype, seemingly independent of changes in MgCYP51 coding sequence. Characterised by a 7-16-fold reduction in in vitro sensitivity to all azoles tested and by growth on seedlings at higher doses of azoles in glasshouse tests compared with isolates carrying the same MgCYP51 variant (L50S, S188N, I381V, ΔY459/G460, N513K), isolates with this phenotype constitutively overexpress MgCYP51 by between 10- and 40-fold compared with the wild type. Analysis of sequences upstream of the predicted MgCYP51 translation start codon identified a novel 120 bp indel, considered to be an insertion, in isolates overexpressing MgCYP51.The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.
|
Antifungal activity against boscalid-resistant Alternaria alternata isolate Aa122 expressing AaSDHC H134R mutant
|
Alternaria alternata
|
50.0
ppm
|
|
Journal : Crop Protection
Year : 2010
Volume : 29
Issue : 7
First Page : 643
Last Page : 651
|
Antifungal activity against boscalid-resistant Alternaria alternata isolate Aa111 expressing AaSDHB H277Y mutant
|
Alternaria alternata
|
50.0
ppm
|
|
Journal : Crop Protection
Year : 2010
Volume : 29
Issue : 7
First Page : 643
Last Page : 651
|
Antifungal activity against boscalid-resistant Alternaria alternata isolate Aa29 expressing AaSDHB H277Y mutant
|
Alternaria alternata
|
50.0
ppm
|
|
Journal : Crop Protection
Year : 2010
Volume : 29
Issue : 7
First Page : 643
Last Page : 651
|
Antifungal activity against wild type Alternaria alternata isolate Aa16
|
Alternaria alternata
|
0.5
ppm
|
|
Journal : Crop Protection
Year : 2010
Volume : 29
Issue : 7
First Page : 643
Last Page : 651
|
Antifungal activity against boscalid-resistant Alternaria alternata expressing AaSDHD H133R mutant assessed as inhibition of mycelial growth at 10 ppm relative to untreated control
|
Alternaria alternata
|
47.46
%
|
|
Journal : Crop Protection
Year : 2010
Volume : 29
Issue : 7
First Page : 643
Last Page : 651
|
Antifungal activity against boscalid-resistant Alternaria alternata expressing AaSDHC H134R mutant assessed as inhibition of mycelial growth at 10 ppm relative to untreated control
|
Alternaria alternata
|
31.87
%
|
|
Journal : Crop Protection
Year : 2010
Volume : 29
Issue : 7
First Page : 643
Last Page : 651
|
Antifungal activity against boscalid-resistant Alternaria alternata expressing AaSDHB H277Y mutant assessed as inhibition of mycelial growth at 10 ppm relative to untreated control
|
Alternaria alternata
|
30.7
%
|
|
Journal : Crop Protection
Year : 2010
Volume : 29
Issue : 7
First Page : 643
Last Page : 651
|
Antifungal activity against wild type Alternaria alternata assessed as inhibition of mycelial growth at 10 ppm relative to untreated control
|
Alternaria alternata
|
87.23
%
|
|
Journal : Crop Protection
Year : 2010
Volume : 29
Issue : 7
First Page : 643
Last Page : 651
|
Antifungal activity against resistant Botryotinia fuckeliana isolates obtained from excessive treatment with with Signum
|
Botryotinia fuckeliana
|
100.0
ppm
|
|
Journal : Crop Protection
Year : 2010
Volume : 29
Issue : 7
First Page : 643
Last Page : 651
|
Antifungal activity against Botryotinia fuckeliana inoculated in strawberry fruit
|
Botryotinia fuckeliana
|
0.09
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Curative antifungal activity against Botryotinia fuckeliana inoculated in strawberry fruit assessed as disease control efficacy at 300 ug AI/ml applied 96 hr post-inoculation relative to control
|
Botryotinia fuckeliana
|
29.8
%
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Curative antifungal activity against Botryotinia fuckeliana inoculated in strawberry fruit assessed as disease severity measuring lesion diameter at 300 ug AI/ml applied 96 hr post-inoculation
|
Botryotinia fuckeliana
|
13.2
mm
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Curative antifungal activity against Botryotinia fuckeliana inoculated in strawberry fruit assessed as disease control efficacy at 300 ug AI/ml applied 24 hr post-inoculation relative to control
|
Botryotinia fuckeliana
|
74.7
%
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Curative antifungal activity against Botryotinia fuckeliana inoculated in strawberry fruit assessed as disease severity measuring lesion diameter at 300 ug AI/ml applied 24 hr post-inoculation
|
Botryotinia fuckeliana
|
4.7
mm
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Curative antifungal activity against Botryotinia fuckeliana inoculated in strawberry fruit assessed as disease control efficacy at 300 ug AI/ml applied 48 hr post-inoculation relative to control
|
Botryotinia fuckeliana
|
94.1
%
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Curative antifungal activity against Botryotinia fuckeliana inoculated in strawberry fruit assessed as disease severity measuring lesion diameter at 300 ug AI/ml applied 48 hr post-inoculation
|
Botryotinia fuckeliana
|
1.1
mm
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Protective antifungal activity against Botryotinia fuckeliana inoculated in fungicide-applied strawberry fruit assessed as disease control efficacy at 300 ug AI/ml applied 24 hr prior inoculation relative to control
|
Botryotinia fuckeliana
|
91.7
%
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Protective antifungal activity against Botryotinia fuckeliana inoculated in fungicide-applied strawberry fruit assessed as disease severity measuring lesion diameter at 300 ug AI/ml applied 24 hr prior inoculation
|
Botryotinia fuckeliana
|
1.5
mm
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Protective antifungal activity against Botryotinia fuckeliana inoculated in fungicide-applied strawberry fruit assessed as disease control efficacy at 300 ug AI/ml applied 48 hr prior inoculation relative to control
|
Botryotinia fuckeliana
|
84.0
%
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Protective antifungal activity against Botryotinia fuckeliana inoculated in fungicide-applied strawberry fruit assessed as disease severity measuring lesion diameter at 300 ug AI/ml applied 48 hr prior inoculation
|
Botryotinia fuckeliana
|
3.0
mm
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
|
Protective antifungal activity against Botryotinia fuckeliana inoculated in fungicide-applied strawberry fruit assessed as disease control efficacy at 300 ug AI/ml applied 96 hr prior-inoculation relative to control
|
Botryotinia fuckeliana
|
81.8
%
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
