|
Adulticidal activity against female Bemisia tabaci biotype B (sweet potato whitefly) in cucumber leaves assessed as residual contact toxicity treated for 30 secs before adult insect infestation measured after 24 hr by leaf dip bioassay
|
Bemisia tabaci
|
336.6
ppm
|
|
Journal : J Agric Food Chem
Year : 2011
Volume : 59
Issue : 15
First Page : 8193
Last Page : 8198
|
Biotype susceptibility ratio, ratio of LC50 for female adult Bemisia tabaci biotype Q (sweet potato whitefly) to LC50 for female adult Bemisia tabaci biotype B
|
Bemisia tabaci
|
1.4
|
|
Journal : J Agric Food Chem
Year : 2011
Volume : 59
Issue : 15
First Page : 8193
Last Page : 8198
|
Adulticidal activity against female Bemisia tabaci biotype Q (sweet potato whitefly) in cucumber leaves assessed as residual contact toxicity treated for 30 secs before adult insect infestation measured after 24 hr by leaf dip bioassay
|
Bemisia tabaci
|
481.7
ppm
|
|
Journal : J Agric Food Chem
Year : 2011
Volume : 59
Issue : 15
First Page : 8193
Last Page : 8198
|
Potency index, ratio of LC50 for cypermethrin to LC50 for test compound against Culex pipiens pallens (mosquito) fourth-instar larvae assessed as insect mortality measured 24 hr post compound exposure by dipping method
|
Culex pipiens pallens
|
100.0
|
|
Journal : J Agric Food Chem
Year : 2011
Volume : 59
Issue : 4
First Page : 1171
Last Page : 1177
|
Insecticidal activity against Culex pipiens pallens (mosquito) fourth-instar larvae assessed as insect mortality measured 24 hr post compound exposure by dipping method
|
Culex pipiens pallens
|
0.0286
mg/L
|
|
Insecticidal activity against Culex pipiens pallens (mosquito) fourth-instar larvae assessed as insect mortality measured 24 hr post compound exposure by dipping method
|
Culex pipiens pallens
|
0.0106
ug.mL-1
|
|
Journal : J Agric Food Chem
Year : 2011
Volume : 59
Issue : 4
First Page : 1171
Last Page : 1177
|
Insecticidal activity against third-instar larval stage of prestarved Spodoptera litura in castor leaf assessed as mortality at 0.1% treated for 2 secs before larval infestation measured after 24 hr by feeding method
|
Spodoptera litura
|
100.0
%
|
|
Journal : J Agric Food Chem
Title : Synthesis of nalidixic acid based hydrazones as novel pesticides.
Year : 2010
Volume : 58
Issue : 5
First Page : 3056
Last Page : 3061
Authors : Aggarwal N, Kumar R, Srivastva C, Dureja P, Khurana JM.
Abstract : Thirty-one substituted hydrazones of nalidixic acid hydrazide were synthesized and characterized by spectral techniques. These compounds were evaluated for various biological activities, namely, fungicidal, insecticidal, and nitrification inhibitory activities. The antifungal activity was evaluated against five pathogenic fungi, namely, Rhizoctonia bataticola , Sclerotium rolfsii , Rhizoctonia solani , Fusarium oxysporum , and Alternaria porii . They showed maximum inihibition against A. porii with ED(50) = 34.2-151.3 microg/mL. The activity was comparable to that of a commercial fungicide, hexaconazole (ED(50) = 25.4 microg/mL). They were also screened for insecticidal activity against third-instar larvae of Spodoptera litura and adults of Callosobruchus maculatus and Tribollium castaneum . Most of them showed 70-100% mortality against S. litura through feeding method at 0.1% dose. These compounds were not found to be effective nitrification inhibitors.
|
Insecticidal activity against Trichoplusia ni (cabbage looper) second-instar larvae infested on compound pre-treated cabbage leaf disk assessed as insect mortality measured 5 days post compound treatment under green house conditions
|
Trichoplusia ni
|
0.6
ppm
|
|
Journal : J Agric Food Chem
Year : 2007
Volume : 55
Issue : 18
First Page : 7517
Last Page : 7526
|
Insecticidal activity against Spodoptera exigua second-instar larvae feeded on compound treated lepidopteran diet assessed as insect mortality measured 5 days post compound treatment under green house conditions
|
Spodoptera exigua
|
1.6
ppm
|
|
Journal : J Agric Food Chem
Year : 2007
Volume : 55
Issue : 18
First Page : 7517
Last Page : 7526
|
Insecticidal activity against Heliothis virescens (tobacco budworm) second-instar larvae infested on compound pre-treated cotton cotyledon disk assessed as insect mortality measured 5 days post compound treatment under green house conditions
|
Heliothis virescens
|
1.3
ppm
|
|
Journal : J Agric Food Chem
Year : 2007
Volume : 55
Issue : 18
First Page : 7517
Last Page : 7526
|
Risk quotient, recommended field rate (g/ha) to LC50 for Trichogramma nubilale (mg/L)
|
Trichogramma nubilale
|
4.62
|
|
Journal : Crop Protection
Title : Susceptibility of adult Trichogramma nubilale (Hymenoptera: Trichogrammatidae) to selected insecticides with different modes of action
Year : 2012
Volume : 34
First Page : 76
Last Page : 72
Authors : Wang Y, Yu R, Zhao X, Chen L, Wu C, Cang T, Wang Q.
Abstract : The parasitic wasp Trichogramma nubilale (Hymenoptera: Trichogrammatidae) is one of the most suitable parasitoids for controlling Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). Although extensive toxicological tests have been carried out to elucidate the toxicities of insecticides to trichogrammatids, the acute toxicity risks of commonly used insecticides to T. nubilale are not well known. Among the 7 classes of tested chemicals, organophosphates and carbamates had the highest intrinsic toxicity to the parasitoid with LC50 values ranging from 0.081 (0.062–0.12) to 2.10 (1.23–3.47) and from 0.12 (0.11–0.14) to 0.95 (0.87–1.05) mg a.i. per liter, respectively. The phenylpyrazoles (with the exception of butene-fipronil), avermectins, neonicotinoids and pyrethroids induced intermediate toxicity responses with LC50 values ranging from 0.29 to 4.67, 2.36 to 11.27, 1.86 to 311.9, and 10.98–150.3 mg a.i. per liter, respectively. In contrast, insect growth regulators (IGRs) exhibited the least toxicity to the parasitoid with LC50 values ranging from 3452 (3114–3877) to 10,168 (8848–12,027) mg a.i. per liter. A risk quotient analysis indicated that neonicotinoids, avermectins, pyrethroids, IGRs and phenylpyrazoles (with the exception of butene-fipronil) were safe, but organophosphates and carbamates were slightly to moderately toxic or highly toxic to T. nubilale. This study provided informative data for implementing both biological and chemical control strategies in integrated pest management (IPM) of corn lepidopterans.
|
Contact toxicity against Trichogramma nubilale assessed as mortality after 24 hr by modified slight dry film method
|
Trichogramma nubilale
|
176.8
mgAi/L
|
|
Contact toxicity against Trichogramma nubilale assessed as mortality after 24 hr by modified slight dry film method
|
Trichogramma nubilale
|
19.48
mgAi/L
|
|
Journal : Crop Protection
Title : Susceptibility of adult Trichogramma nubilale (Hymenoptera: Trichogrammatidae) to selected insecticides with different modes of action
Year : 2012
Volume : 34
First Page : 76
Last Page : 72
Authors : Wang Y, Yu R, Zhao X, Chen L, Wu C, Cang T, Wang Q.
Abstract : The parasitic wasp Trichogramma nubilale (Hymenoptera: Trichogrammatidae) is one of the most suitable parasitoids for controlling Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). Although extensive toxicological tests have been carried out to elucidate the toxicities of insecticides to trichogrammatids, the acute toxicity risks of commonly used insecticides to T. nubilale are not well known. Among the 7 classes of tested chemicals, organophosphates and carbamates had the highest intrinsic toxicity to the parasitoid with LC50 values ranging from 0.081 (0.062–0.12) to 2.10 (1.23–3.47) and from 0.12 (0.11–0.14) to 0.95 (0.87–1.05) mg a.i. per liter, respectively. The phenylpyrazoles (with the exception of butene-fipronil), avermectins, neonicotinoids and pyrethroids induced intermediate toxicity responses with LC50 values ranging from 0.29 to 4.67, 2.36 to 11.27, 1.86 to 311.9, and 10.98–150.3 mg a.i. per liter, respectively. In contrast, insect growth regulators (IGRs) exhibited the least toxicity to the parasitoid with LC50 values ranging from 3452 (3114–3877) to 10,168 (8848–12,027) mg a.i. per liter. A risk quotient analysis indicated that neonicotinoids, avermectins, pyrethroids, IGRs and phenylpyrazoles (with the exception of butene-fipronil) were safe, but organophosphates and carbamates were slightly to moderately toxic or highly toxic to T. nubilale. This study provided informative data for implementing both biological and chemical control strategies in integrated pest management (IPM) of corn lepidopterans.
|
Insecticidal activity against Spodoptera litura third-instar larvae exposed to 0.1% compound on ventral side of larvae assessed as mortality after 24 hr post compound treatment by contact method
|
Spodoptera litura
|
100.0
%
|
|
Journal : Eur. J. Med. Chem.
Year : 2012
Volume : 58
First Page : 470
Last Page : 477
|
Synergism factor, ratio of LD50 for fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR08 to LD50 for fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR08 in presence of 3.2 microg/larva propenofos
|
Helicoverpa armigera
|
3.7
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Synergism factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Gaschiga isolated form hyptis plant to LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Gaschiga isolated from hyptis plant in presence of 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO
|
Helicoverpa armigera
|
25.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Synergism factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Ngong to LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Ngong in presence of 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO
|
Helicoverpa armigera
|
37.5
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Synergism factor, ratio of LD50 for Helicoverpa armigera field strain Guider to LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Guider in presence of 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO
|
Helicoverpa armigera
|
3.6
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Synergism factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera Foumbot to LD50 for fourth-instar larval stage of Helicoverpa armigera Foumbot in presence of 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO
|
Helicoverpa armigera
|
1.3
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Synergism factor, ratio of LD50 for fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera field strain Pitoa isolated from tomato plant to LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Pitoa isolated from tomato plant in presence of 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO
|
Helicoverpa armigera
|
5.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Synergism factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Pitoa isolated from maize plant to LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Pitoa isolated from maize plant in presence of 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO
|
Helicoverpa armigera
|
8.3
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Synergism factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Home to LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Home in presence of 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO
|
Helicoverpa armigera
|
2.1
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Synergism factor, ratio of LD50 for fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR06 to LD50 for fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR06 in presence of 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO
|
Helicoverpa armigera
|
1.5
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR08 assessed as mortality applied topically onto thorax measured after 48 hr in presence of 3.2 microg/larva propenofos
|
Helicoverpa armigera
|
226.6
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Gaschiga isolated from hyptis plant assessed as mortality applied topically onto thorax 30 min after 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO application measured after 48 hr
|
Helicoverpa armigera
|
1.8
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Ngong isolated from cotton plant assessed as mortality applied topically onto thorax 30 min after 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO application measured after 48 hr
|
Helicoverpa armigera
|
3.0
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Guider isolated from cotton plant assessed as mortality applied topically onto thorax 30 min after 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO application measured after 48 hr
|
Helicoverpa armigera
|
8.8
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera Foumbot isolated from tomato plant assessed as mortality applied topically onto thorax 30 min after 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO application measured after 48 hr
|
Helicoverpa armigera
|
63.0
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Pitoa isolated from tomato plant assessed as mortality applied topically onto thorax 30 min after 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO application measured after 48 hr
|
Helicoverpa armigera
|
53.2
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Pitoa isolated from maize plant assessed as mortality applied topically onto thorax 30 min after 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO application measured after 48 hr
|
Helicoverpa armigera
|
28.9
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Home isolated from cotton plant assessed as mortality applied topically onto thorax 30 min after 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO application measured after 48 hr
|
Helicoverpa armigera
|
21.3
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR06 assessed as mortality applied topically onto thorax 30 min after 10 microg/larva cytochrome P450 monooxygenase inhibitor, PBO application measured after 48 hr
|
Helicoverpa armigera
|
480.3
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Gaschiga isolated from hyptis plant to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
96.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Ngong to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
240.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Djalingo to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
194.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Guider to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
67.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Mokong to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
152.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera Foumbot to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
174.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Tchollire to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
102.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Pitoa isolated from maize plant to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
510.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Sorga to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
123.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of Helicoverpa armigera field strain Home to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
93.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR08 to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
1771.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LD50 for fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR06 to LD50 for fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77
|
Helicoverpa armigera
|
1529.0
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Gaschiga isolated from hyptis plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
45.0
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Djalingo isolated from cotton plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
91.5
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Ngong isolated from cotton plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
112.6
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Guider isolated from cotton plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
31.6
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Mokong isolated from cotton plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
71.5
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera Foumbot isolated from tomato plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
81.6
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Pitoa isolated from maize plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
239.9
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Sorga isolated from cotton plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
57.7
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Tchollire isolated from cotton plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
48.0
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of Helicoverpa armigera field strain Home isolated from cotton plant assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
43.9
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR08 assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
832.5
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of pyrethroid-resistance Helicoverpa armigera laboratory strain GS-RR06 assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
718.4
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Insecticidal activity against fourth-instar larval stage of pyrethroid-susceptible Helicoverpa armigera laboratory strain BK-77 assessed as mortality applied topically onto thorax measured after 48 hr
|
Helicoverpa armigera
|
0.5
microgAi/g
|
|
Journal : Pest Manag Sci
Title : Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa.
Year : 2009
Volume : 65
Issue : 10
First Page : 1147
Last Page : 1154
Authors : Achaleke J, Martin T, Ghogomu RT, Vaissayre M, Brévault T.
Abstract : BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67-1771), cross-resistance to deltamethrin (RF = 60-2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2-10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes.
|
Resistance factor, ratio of LC50 for adult female Bemisia tabaci B-biotype NJ-Imi (sweet potato whitefly) (G30) to LC50 for adult female B-type Bemisia tabaci NJ
|
Bemisia tabaci
|
1.0
|
|
Journal : Pest Manag Sci
Title : Cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in B-biotype Bemisia tabaci.
Year : 2009
Volume : 65
Issue : 11
First Page : 1189
Last Page : 1194
Authors : Wang Z, Yao M, Wu Y.
Abstract : BACKGROUND: The B-type Bemisia tabaci (Gennadius) has become established in many regions in China, and neonicotinoids are extensively used to control this pest. Imidacloprid resistance in a laboratory-selected strain of B-type B. tabaci was characterised in order to provide the basis for recommending resistance management tactics. RESULTS: The NJ-Imi strain of B-type B. tabaci was selected from the NJ strain with imidacloprid for 30 generations. The NJ-Imi strain exhibited 490-fold resistance to imidacloprid, high levels of cross-resistance to three other neonicotinoids, low levels of cross-resistance to monosultap, cartap and spinosad, but no cross-resistance to abamectin and cypermethrin. Imidacloprid resistance in the NJ-Imi strain was autosomal and semi-dominant. It is shown that enhanced detoxification mediated by cytochrome-P450-dependent monooxygenases contributes to imidacloprid resistance to some extent in the NJ-Imi strain. Results from synergist bioassays and cross-resistance patterns indicated that target-site insensitivity may be involved in imidacloprid resistance in the NJ-Imi strain of B. tabaci. CONCLUSION: Although oxidative detoxification mediated by P450 monooxygenases is involved in imidacloprid resistance in the NJ-Imi strain of B-type B. tabaci, target-site modification as an additional resistance mechanism cannot be ruled out. Considering the high risk of cross-resistance, neonicotinoids should be regarded as a single group when implementing an insecticide rotation scheme in B. tabaci control.
|
Resistance factor, ratio of LC50 for adult female Bemisia tabaci B-biotype NJ-Imi (sweet potato whitefly) (G15) to LC50 for adult female B-type Bemisia tabaci NJ
|
Bemisia tabaci
|
1.0
|
|
Journal : Pest Manag Sci
Title : Cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in B-biotype Bemisia tabaci.
Year : 2009
Volume : 65
Issue : 11
First Page : 1189
Last Page : 1194
Authors : Wang Z, Yao M, Wu Y.
Abstract : BACKGROUND: The B-type Bemisia tabaci (Gennadius) has become established in many regions in China, and neonicotinoids are extensively used to control this pest. Imidacloprid resistance in a laboratory-selected strain of B-type B. tabaci was characterised in order to provide the basis for recommending resistance management tactics. RESULTS: The NJ-Imi strain of B-type B. tabaci was selected from the NJ strain with imidacloprid for 30 generations. The NJ-Imi strain exhibited 490-fold resistance to imidacloprid, high levels of cross-resistance to three other neonicotinoids, low levels of cross-resistance to monosultap, cartap and spinosad, but no cross-resistance to abamectin and cypermethrin. Imidacloprid resistance in the NJ-Imi strain was autosomal and semi-dominant. It is shown that enhanced detoxification mediated by cytochrome-P450-dependent monooxygenases contributes to imidacloprid resistance to some extent in the NJ-Imi strain. Results from synergist bioassays and cross-resistance patterns indicated that target-site insensitivity may be involved in imidacloprid resistance in the NJ-Imi strain of B. tabaci. CONCLUSION: Although oxidative detoxification mediated by P450 monooxygenases is involved in imidacloprid resistance in the NJ-Imi strain of B-type B. tabaci, target-site modification as an additional resistance mechanism cannot be ruled out. Considering the high risk of cross-resistance, neonicotinoids should be regarded as a single group when implementing an insecticide rotation scheme in B. tabaci control.
|
Insecticidal activity against adult female imidacloprid-resistant Bemisia tabaci B-biotype NJ-Imi (sweet potato whitefly) (G30) in cotton leaf discs assessed as mortality treated for 10 secs prior to adult infestation measured after 72 hr by leaf-dip bioassay
|
Bemisia tabaci
|
263.0
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in B-biotype Bemisia tabaci.
Year : 2009
Volume : 65
Issue : 11
First Page : 1189
Last Page : 1194
Authors : Wang Z, Yao M, Wu Y.
Abstract : BACKGROUND: The B-type Bemisia tabaci (Gennadius) has become established in many regions in China, and neonicotinoids are extensively used to control this pest. Imidacloprid resistance in a laboratory-selected strain of B-type B. tabaci was characterised in order to provide the basis for recommending resistance management tactics. RESULTS: The NJ-Imi strain of B-type B. tabaci was selected from the NJ strain with imidacloprid for 30 generations. The NJ-Imi strain exhibited 490-fold resistance to imidacloprid, high levels of cross-resistance to three other neonicotinoids, low levels of cross-resistance to monosultap, cartap and spinosad, but no cross-resistance to abamectin and cypermethrin. Imidacloprid resistance in the NJ-Imi strain was autosomal and semi-dominant. It is shown that enhanced detoxification mediated by cytochrome-P450-dependent monooxygenases contributes to imidacloprid resistance to some extent in the NJ-Imi strain. Results from synergist bioassays and cross-resistance patterns indicated that target-site insensitivity may be involved in imidacloprid resistance in the NJ-Imi strain of B. tabaci. CONCLUSION: Although oxidative detoxification mediated by P450 monooxygenases is involved in imidacloprid resistance in the NJ-Imi strain of B-type B. tabaci, target-site modification as an additional resistance mechanism cannot be ruled out. Considering the high risk of cross-resistance, neonicotinoids should be regarded as a single group when implementing an insecticide rotation scheme in B. tabaci control.
|
Insecticidal activity against adult female imidacloprid-resistant Bemisia tabaci B-biotype NJ-Imi (sweet potato whitefly) (G15) in cotton leaf discs assessed as mortality treated for 10 secs prior to adult infestation measured after 72 hr by leaf-dip bioassay
|
Bemisia tabaci
|
230.0
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in B-biotype Bemisia tabaci.
Year : 2009
Volume : 65
Issue : 11
First Page : 1189
Last Page : 1194
Authors : Wang Z, Yao M, Wu Y.
Abstract : BACKGROUND: The B-type Bemisia tabaci (Gennadius) has become established in many regions in China, and neonicotinoids are extensively used to control this pest. Imidacloprid resistance in a laboratory-selected strain of B-type B. tabaci was characterised in order to provide the basis for recommending resistance management tactics. RESULTS: The NJ-Imi strain of B-type B. tabaci was selected from the NJ strain with imidacloprid for 30 generations. The NJ-Imi strain exhibited 490-fold resistance to imidacloprid, high levels of cross-resistance to three other neonicotinoids, low levels of cross-resistance to monosultap, cartap and spinosad, but no cross-resistance to abamectin and cypermethrin. Imidacloprid resistance in the NJ-Imi strain was autosomal and semi-dominant. It is shown that enhanced detoxification mediated by cytochrome-P450-dependent monooxygenases contributes to imidacloprid resistance to some extent in the NJ-Imi strain. Results from synergist bioassays and cross-resistance patterns indicated that target-site insensitivity may be involved in imidacloprid resistance in the NJ-Imi strain of B. tabaci. CONCLUSION: Although oxidative detoxification mediated by P450 monooxygenases is involved in imidacloprid resistance in the NJ-Imi strain of B-type B. tabaci, target-site modification as an additional resistance mechanism cannot be ruled out. Considering the high risk of cross-resistance, neonicotinoids should be regarded as a single group when implementing an insecticide rotation scheme in B. tabaci control.
|
Insecticidal activity against adult female imidacloprid-susceptible Bemisia tabaci B-biotype NJ (sweet potato whitefly) in cotton leaf discs assessed as mortality treated for 10 secs prior to adult infestation measured after 72 hr by leaf-dip bioassay
|
Bemisia tabaci
|
226.0
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in B-biotype Bemisia tabaci.
Year : 2009
Volume : 65
Issue : 11
First Page : 1189
Last Page : 1194
Authors : Wang Z, Yao M, Wu Y.
Abstract : BACKGROUND: The B-type Bemisia tabaci (Gennadius) has become established in many regions in China, and neonicotinoids are extensively used to control this pest. Imidacloprid resistance in a laboratory-selected strain of B-type B. tabaci was characterised in order to provide the basis for recommending resistance management tactics. RESULTS: The NJ-Imi strain of B-type B. tabaci was selected from the NJ strain with imidacloprid for 30 generations. The NJ-Imi strain exhibited 490-fold resistance to imidacloprid, high levels of cross-resistance to three other neonicotinoids, low levels of cross-resistance to monosultap, cartap and spinosad, but no cross-resistance to abamectin and cypermethrin. Imidacloprid resistance in the NJ-Imi strain was autosomal and semi-dominant. It is shown that enhanced detoxification mediated by cytochrome-P450-dependent monooxygenases contributes to imidacloprid resistance to some extent in the NJ-Imi strain. Results from synergist bioassays and cross-resistance patterns indicated that target-site insensitivity may be involved in imidacloprid resistance in the NJ-Imi strain of B. tabaci. CONCLUSION: Although oxidative detoxification mediated by P450 monooxygenases is involved in imidacloprid resistance in the NJ-Imi strain of B-type B. tabaci, target-site modification as an additional resistance mechanism cannot be ruled out. Considering the high risk of cross-resistance, neonicotinoids should be regarded as a single group when implementing an insecticide rotation scheme in B. tabaci control.
|
Resistant ratio, ratio of LC90 for F1 generation of adult of Helicoverpa zea (corn earworm) to LC90 for adult of corn earworm
|
Helicoverpa zea
|
3.82
|
|
Journal : Pest Manag Sci
Year : 2010
Volume : 66
Issue : 2
First Page : 147
Last Page : 154
|
Resistant ratio, ratio of LC90 for F1 generation of third-instar of Helicoverpa zea (corn earworm) to LC90 for third-instar of corn earworm
|
Helicoverpa zea
|
6.28
|
|
Journal : Pest Manag Sci
Year : 2010
Volume : 66
Issue : 2
First Page : 147
Last Page : 154
|
Resistant ratio, ratio of LC50 for F1 generation of adult of Helicoverpa zea (corn earworm) to LC50 for adult of corn earworm
|
Helicoverpa zea
|
4.16
|
|
Journal : Pest Manag Sci
Year : 2010
Volume : 66
Issue : 2
First Page : 147
Last Page : 154
|
Resistant ratio, ratio of LC50 for F1 generation of third-instar of Helicoverpa zea (corn earworm) to LC50 for third-instar of corn earworm
|
Helicoverpa zea
|
4.68
|
|
Journal : Pest Manag Sci
Year : 2010
Volume : 66
Issue : 2
First Page : 147
Last Page : 154
|
Insecticidal activity against F1 generation of adult of Helicoverpa zea (corn earworm) assessed as mortality per insecticide vial after 24 hr
|
Helicoverpa zea
|
7.12
ug
|
|
Insecticidal activity against F1 generation of adult of Helicoverpa zea (corn earworm) assessed as mortality per insecticide vial after 24 hr
|
Helicoverpa zea
|
2.89
ug
|
|
Journal : Pest Manag Sci
Year : 2010
Volume : 66
Issue : 2
First Page : 147
Last Page : 154
|
Insecticidal activity against F1 generation of third-instar of Helicoverpa zea (corn earworm) assessed as mortality per insecticide vial after 48 hr
|
Helicoverpa zea
|
7.68
ug
|
|
Insecticidal activity against F1 generation of third-instar of Helicoverpa zea (corn earworm) assessed as mortality per insecticide vial after 48 hr
|
Helicoverpa zea
|
1.71
ug
|
|
Journal : Pest Manag Sci
Year : 2010
Volume : 66
Issue : 2
First Page : 147
Last Page : 154
|
Insecticidal activity against adult of Helicoverpa zea (corn earworm) assessed as mortality per insecticide vial after 24 hr
|
Helicoverpa zea
|
1.86
ug
|
|
Insecticidal activity against adult of Helicoverpa zea (corn earworm) assessed as mortality per insecticide vial after 24 hr
|
Helicoverpa zea
|
0.7
ug
|
|
Journal : Pest Manag Sci
Year : 2010
Volume : 66
Issue : 2
First Page : 147
Last Page : 154
|
Insecticidal activity against third-instar of Helicoverpa zea (corn earworm) assessed as mortality per insecticide vial after 48 hr
|
Helicoverpa zea
|
1.22
ug
|
|
Insecticidal activity against third-instar of Helicoverpa zea (corn earworm) assessed as mortality per insecticide vial after 48 hr
|
Helicoverpa zea
|
0.37
ug
|
|
Journal : Pest Manag Sci
Year : 2010
Volume : 66
Issue : 2
First Page : 147
Last Page : 154
|
Induction of cell growth in Spodoptera frugiperda (fall armyworm) Sf9 cells at 10 uM after 72 hr by trypan blue dye exclusion test
|
Spodoptera frugiperda
|
None
|
|
Journal : J Pesticide Sci
Title : Effects of Pyridalyl, a Novel Insecticidal Agent, on Cultured Sf9 Cells
Year : 2005
Volume : 30
Issue : 1
First Page : 17
Last Page : 21
Authors : Saito S, Sakamoto N, Umeda K
|
Synergist ratio, ratio of LC50 for Aphis gossypii clone Burk (cotton aphid) in presence of S,S,S-tri-n-butyl phosphorotrithioate to LC50 for Aphis gossypii clone Burk
|
Aphis gossypii
|
1.3
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Synergist ratio, ratio of LC50 for Aphis gossypii clone Burk (cotton aphid) in presence of piperonyl butoxide to LC50 for Aphis gossypii clone NM1
|
Aphis gossypii
|
1.0
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Synergist ratio, ratio of LC50 for Aphis gossypii clone NM1 (cotton aphid) in presence of S,S,S-tri-n-butyl phosphorotrithioate to LC50 for Aphis gossypii clone NM1
|
Aphis gossypii
|
1.3
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Synergist ratio, ratio of LC50 for Aphis gossypii clone NM1 (cotton aphid) in presence of piperonyl butoxide to LC50 for Aphis gossypii clone NM1
|
Aphis gossypii
|
0.8
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Synergist ratio, ratio of LC50 for Aphis gossypii clone Auber (cotton aphid) in presence of piperonyl butoxide to LC50 for Aphis gossypii clone Auber
|
Aphis gossypii
|
4.2
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Synergist ratio, ratio of LC50 for Aphis gossypii clone Auber (cotton aphid) in presence of S,S,S-tri-n-butyl phosphorotrithioate to LC50 for Aphis gossypii clone Auber
|
Aphis gossypii
|
2.4
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone Burk (cotton aphid) in egg plant leaf assessed as mortality after 24 hr by leaf disk assay in presence of S,S,S-tri-n-butyl phosphorotrithioate
|
Aphis gossypii
|
18516.0
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone Burk (cotton aphid) in egg plant leaf assessed as mortality after 24 hr by leaf disk assay in presence of piperonyl butoxide
|
Aphis gossypii
|
207.0
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone Auber (cotton aphid) in egg plant leaf assessed as mortality after 24 hr by leaf disk assay in presence of piperonyl butoxide
|
Aphis gossypii
|
23360.0
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone Auber (cotton aphid) in egg plant leaf assessed as mortality after 24 hr by leaf disk assay in presence of S,S,S-tri-n-butyl phosphorotrithioate
|
Aphis gossypii
|
8.5
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone NM1 (cotton aphid) in zucchini or squash leaf assessed as mortality after 24 hr by leaf disk assay in presence of S,S,S-tri-n-butyl phosphorotrithioate
|
Aphis gossypii
|
119.0
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone NM1 (cotton aphid) in zucchini or squash leaf assessed as mortality after 24 hr by leaf disk assay in presence of piperonyl butoxide
|
Aphis gossypii
|
13.2
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Resistant factor, ratio of LC50 for Aphis gossypii clone Ivo (cotton aphid) to Aphis gossypii clone NM1
|
Aphis gossypii
|
0.5
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Resistant factor, ratio of LC50 for Aphis gossypii clone Burk (cotton aphid) to Aphis gossypii clone NM1
|
Aphis gossypii
|
473.0
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Resistant factor, ratio of LC50 for Aphis gossypii clone Auber (cotton aphid) to Aphis gossypii clone NM1
|
Aphis gossypii
|
41.2
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Resistant factor, ratio of LC50 for Aphis gossypii clone PsP4 (cotton aphid) to Aphis gossypii clone NM1
|
Aphis gossypii
|
1.4
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Resistant factor, ratio of LC50 for Aphis gossypii clone C4 (cotton aphid) to Aphis gossypii clone NM1
|
Aphis gossypii
|
0.4
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Resistant factor, ratio of LC50 for Aphis gossypii clone C9 (cotton aphid) to Aphis gossypii clone NM1
|
Aphis gossypii
|
0.5
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone Ivo (cotton aphid) in cotton plant leaf assessed as mortality after 24 hr by leaf disk assay
|
Aphis gossypii
|
7.1
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone Burk (cotton aphid) in cotton plant leaf assessed as mortality after 24 hr by leaf disk assay
|
Aphis gossypii
|
6763.5
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone Auber (cotton aphid) in egg plant leaf assessed as mortality after 24 hr by leaf disk assay
|
Aphis gossypii
|
504.0
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone PsP4 (cotton aphid) in pepper plant leaf assessed as mortality after 24 hr by leaf disk assay
|
Aphis gossypii
|
19.7
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone C4 (cotton aphid) in zucchini or squash leaf assessed as mortality after 24 hr by leaf disk assay
|
Aphis gossypii
|
6.3
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone C9 (cotton aphid) in zucchini or squash leaf assessed as mortality after 24 hr by leaf disk assay
|
Aphis gossypii
|
7.2
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
|
Insecticidal activity against Aphis gossypii clone NM1 (cotton aphid) in zucchini or squash leaf assessed as mortality after 24 hr by leaf disk assay
|
Aphis gossypii
|
10.9
ugAi/ml
|
|
Journal : Pest Manag Sci
Title : Insecticide resistance traits differ among and within host races in Aphis gossypii.
Year : 2010
Volume : 66
Issue : 3
First Page : 301
Last Page : 307
Authors : Carletto J, Martin T, Vanlerberghe-Masutti F, Brévault T.
Abstract : BACKGROUND: The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance. RESULTS: All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes. CONCLUSION: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
