NOVOBIOCIN

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Synonyms
Status
Molecule Category Free-form
UNII 17EC19951N
EPA CompTox DTXSID3041083

Structure

InChI Key YJQPYGGHQPGBLI-KGSXXDOSSA-N
Smiles CO[C@@H]1[C@@H](OC(N)=O)[C@@H](O)[C@H](Oc2ccc3c(O)c(NC(=O)c4ccc(O)c(CC=C(C)C)c4)c(=O)oc3c2C)OC1(C)C
InChI
InChI=1S/C31H36N2O11/c1-14(2)7-8-16-13-17(9-11-19(16)34)27(37)33-21-22(35)18-10-12-20(15(3)24(18)42-28(21)38)41-29-23(36)25(43-30(32)39)26(40-6)31(4,5)44-29/h7,9-13,23,25-26,29,34-36H,8H2,1-6H3,(H2,32,39)(H,33,37)/t23-,25+,26-,29-/m1/s1

Physicochemical Descriptors

Property Name Value
Molecular Formula C31H36N2O11
Molecular Weight 612.63
AlogP 3.63
Hydrogen Bond Acceptor 11.0
Hydrogen Bond Donor 5.0
Number of Rotational Bond 8.0
Polar Surface Area 200.01
Molecular species ACID
Aromatic Rings 3.0
Heavy Atoms 44.0
Assay Description Organism Bioactivity Reference
Iinhibitory activity against Escherichia coli DNA gyrase Escherichia coli 0.25 ug.mL-1
Inhibitory activity against Escherichia coli DNA gyrase Escherichia coli 0.25 ug.mL-1
Iinhibitory activity against Staphylococcus aureus DNA gyrase Staphylococcus aureus 0.25 ug.mL-1
Iinhibitory activity against Escherichia coli DNA topoisomerase IV Escherichia coli 3.5 ug.mL-1
Iinhibitory activity against Staphylococcus aureus DNA topoisomerase IV Staphylococcus aureus 28.0 ug.mL-1
Iinhibitory activity against human DNA topoisomerase II Homo sapiens 400.0 ug.mL-1
Inhibitory activity against human DNA topoisomerase II None 400.0 ug.mL-1
Inhibitory activity against Escherichia coli DNA topoisomerase IV Escherichia coli 25.0 ug.mL-1
Inhibitory activity against Escherichia coli DNA gyrase Escherichia coli 0.25 ug.mL-1
Percent inhibition of filarial parasite Setaria cervi by compound at 10 ug/mL concentration Setaria cervi 10.0 %
Percent inhibition of filarial parasite Setaria cervi by compound at 20 ug/mL concentration Setaria cervi 20.0 %
Percent inhibition of filarial parasite Setaria cervi by compound at 40 ug/mL concentration Setaria cervi 80.0 %
Inhibition of Staphylococcus aureus DNA gyrase Staphylococcus aureus 10.0 nM
Inhibition of Escherichia coli DNA gyrase Escherichia coli 14.0 nM
Inhibition of Escherichia coli topoisomerase 4 Escherichia coli 110.0 nM
Inhibition of Escherichia coli GyrB ATPase activity assessed as inorganic phosphate release by malachite green complex formation assay Escherichia coli 30.0 nM
Inhibition of Staphylococcus aureus DNA gyrase Staphylococcus aureus 0.25 ug.mL-1
Inhibition of Bacillus subtilis DNA gyrase Bacillus subtilis 0.5 ug.mL-1
Inhibition of Staphylococcus aureus DNA gyrase supercoiling activity Staphylococcus aureus 0.28 ug.mL-1
Inhibition of Escherichia coli DNA gyrase supercoiling activity Escherichia coli 0.31 ug.mL-1
Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in human SH-SY5Y cells assessed as lactate dehydrogenase release Homo sapiens 48.39 nM
Inhibition of Mycobacterium leprae wild type DNA gyrase A2B2 assessed as DNA supercoiling inhibition Mycobacterium leprae 0.25 ug.mL-1
Inhibition of Mycobacterium leprae DNA gyrase subunit A G89C mutant assessed as DNA supercoiling inhibition Mycobacterium leprae 0.3 ug.mL-1
Inhibition of Mycobacterium leprae DNA gyrase subunit A A91V mutant assessed as DNA supercoiling inhibition Mycobacterium leprae 0.2 ug.mL-1
Inhibition of Mycobacterium leprae wild type DNA gyrase subunit B D205N mutant assessed as DNA supercoiling inhibition Mycobacterium leprae 0.3 ug.mL-1
Inhibition of Streptococcus pneumoniae SP-3 gyrB by enzyme coupled phosphate assay Streptococcus pneumoniae 37.0 nM
Antibacterial activity against Staphylococcus aureus UC76 by NCCLS method Staphylococcus aureus 125.0 nM
Inhibition of Escherichia coli DNA gyrase B ATPase activity Escherichia coli 3.0 nM
Inhibition of Escherichia coli DNA gyraseB assessed as inhibition of pBR322 supercoiling by densitometry Escherichia coli 50.0 nM
Inhibition of reconstituted Xanthomonas albilineans DNA gyrase subunit AB assessed as reduction of DNA supercoiling activity using pBR322 DNA substrate Xanthomonas albilineans 800.0 nM
Inhibition of reconstituted Escherichia coli DNA gyrase subunit AB assessed as reduction of DNA supercoiling activity using pBR322 DNA substrate Escherichia coli 100.0 nM
Inhibition of reconstituted Xanthomonas albilineans DNA gyrase A and Escherichia coli DNA gyrase subunit B hybrid assessed as reduction of DNA supercoiling activity using pBR322 DNA substrate None 100.0 nM
Inhibition of Escherichia coli JM109 DNA gyrase subunit B assessed as ATP hydrolysis by ATPase assay Escherichia coli 154.0 nM
Inhibition of Escherichia coli JM109 DNA gyrase subunit B assessed as DNA triple helix formation by supercoiling assay Escherichia coli 200.0 nM
Inhibition of Escherichia coli DNA gyrase by gel electrophoresis Escherichia coli 100.0 nM
Inhibition of human topoisomerase 2alpha-mediated ATP hydrolysis at 400 uM after 30 mins by malachite green ATPase assay Homo sapiens 60.4 %
Inhibition of Escherichia coli DNA gyrase assessed as inhibition of pBR322 supercoiling by densitometry Escherichia coli 50.0 nM
Inhibition of Escherichia coli DNA topoisomerase 4 subunit ParC/DNA topoisomerase 4 subunit ParE-mediated decatenation of kDNA by densitometry Escherichia coli 110.0 nM
Displacement of novobiocin-TRX from Francisella tularensis Schu S4 gyraseB by fluorescence polarization assay Francisella tularensis 8.0 nM
Inhibition of DNA gyrase B None 3.0 nM
Inhibition of Escherichia coli DNA gyrase B using relaxed pNO1 as substrate after 30 mins by fluorimetry Escherichia coli 15.0 nM
Binding affinity to Escherichia coli DNA gyrase subunit B N-terminal fragment G24 measured over 120 secs by surface plasmon resonance method Escherichia coli 28.0 nM
Inhibition of human liver OATP1B1 expressed in HEK293 Flp-In cells assessed as reduction in E17-betaG uptake at 20 uM by scintillation counting Homo sapiens 58.1 %
Inhibition of human liver OATP1B3 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E17-betaG uptake at 20 uM incubated for 5 mins by scintillation counting Homo sapiens 84.2 %
Inhibition of human liver OATP2B1 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E3S uptake at 20 uM incubated for 5 mins by scintillation counting Homo sapiens 85.6 %
Binding affinity to Escherichia coli GyrB Escherichia coli 28.0 nM
Inhibition of Escherichia coli His6-tagged ParC/ParE expressed in baculovirus-infected Sf9 cells by gel electrophoresis Escherichia coli 210.0 nM
Inhibition of Escherichia coli ATPase activity of topoisomerase-4 Escherichia coli 450.0 nM
Inhibition of Escherichia coli ATPase activity of DNA gyrase Escherichia coli 23.0 nM
Inhibition of Staphylococcus aureus DNA gyrase Staphylococcus aureus 4.0 nM
Inhibition of Escherichia coli DNA gyrase Escherichia coli 500.0 nM
Inhibition of human DNA topoisomerase-2alpha ATPase activity using supercoiled pUC18 DNA as substrate assessed as inorganic phosphate release at 400 uM incubated for 30 mins prior to ATP addition measured after 30 mins by malachite green phosphate assay relative to control Homo sapiens 76.8 %
Inhibition of human DNA topoisomerase-2alpha ATPase activity using supercoiled pUC18 DNA as substrate assessed as inorganic phosphate release at 300 uM incubated for 30 mins prior to ATP addition measured after 30 mins by malachite green phosphate assay relative to control Homo sapiens 63.6 %
Inhibition of human DNA topoisomerase-2alpha ATPase activity using supercoiled pUC18 DNA as substrate assessed as inorganic phosphate release at 200 uM incubated for 30 mins prior to ATP addition measured after 30 mins by malachite green phosphate assay relative to control Homo sapiens 14.4 %
Inhibition of Mycobacterium tuberculosis DNA gyrase super coiling activity of relaxed pBR322 using ethidium bromide staining by agarose gel electrophoresis Mycobacterium tuberculosis 50.0 nM
Inhibition of Mycobacterium tuberculosis DNA gyrase super coiling activity of relaxed pBR322 at 25 uM using ethidium bromide staining by agarose gel electrophoresis Mycobacterium tuberculosis 100.0 %
Inhibition of Mycobacterium tuberculosis DNA gyrase super coiling activity of relaxed pBR322 at 50 uM using ethidium bromide staining by agarose gel electrophoresis Mycobacterium tuberculosis 100.0 %
Inhibition of Mycobacterium smegmatis GyrB ATPase activity expressed in Escherichia coli BL21 (DE3) after 2 hrs Mycobacterium smegmatis 200.0 nM
Inhibition of Mycobacterium smegmatis GyrB ATPase activity expressed in Escherichia coli BL21 (DE3) at 25 uM after 2 hrs Mycobacterium smegmatis 96.0 %
Inhibition of Mycobacterium smegmatis GyrB ATPase activity expressed in Escherichia coli BL21 (DE3) at 50 uM after 2 hrs Mycobacterium smegmatis 98.2 %
Inhibition of ACE (unknown origin) assessed as 3-Hydroxybutyril-glycil-glycil-glycine conversion to 3-hydroxybutyric acid at 500 uM after 60 mins by WST assay relative to control Homo sapiens 75.0 %
Inhibition of purified Mycobacterium smegmatis GyrB at 50 to 100 uM after 100 mins by ATPase assay Mycobacterium smegmatis 180.0 nM
Inhibition of Mycobacterium tuberculosis DNA gyrase using relaxed pBR322 as substrate after 30 mins by supercoiling assay Mycobacterium tuberculosis 46.0 nM
Inhibition of Staphylococcus aureus DNA gyrase B ATPase activity Staphylococcus aureus 125.0 nM
Inhibition of Staphylococcus aureus DNA gyrase-mediated supercoiling of relaxed DNA using pBR 322 as substrate by agarose gel electrophoresis analysis Staphylococcus aureus 30.0 nM
Inhibition of Mycobacterium tuberculosis DNA gyrase-mediated relaxed pBR322 DNA supercoiling by gel electrophoresis Mycobacterium tuberculosis 15.0 nM
Cytotoxicity against mouse RAW264.7 cells at 100 uM after 48 hrs by MTT assay Mus musculus 29.44 %
Inhibition of Mycobacterium tuberculosis GyrA/GyrB using relaxed pBR322 as substrate at 0.97 uM after 30 mins by DNA supercoiling assay Mycobacterium tuberculosis 61.0 %
Inhibition of 6-His-tagged Mycobacterium smegmatis GyrB expressed in Escherichia coli BL21 (DE3) pLysS cells incubated for 100 mins in presence of ATP by malachite green dye based ATP assay Mycobacterium smegmatis 180.0 nM
Inhibition of Mycobacterium tuberculosis DNA gyrase supercoiling activity using relaxed pBR322 substrate incubated for 30 mins by ethidium bromide staining method Mycobacterium tuberculosis 46.0 nM
Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell viability at 100 uM after 48 hrs by MTT assay Mus musculus 19.36 %
Inhibition of Mycobacterium tuberculosis DNA GyrA/B heterotetramer assessed as inhibition of pBR322 supercoiling after 30 mins by gel electrophoresis Mycobacterium tuberculosis 180.0 nM
Inhibition of Mycobacterium smegmatis DNA GyrB domain assessed as inhibition of inorganic phosphate release after 100 mins at room temperature by ATPase assay Mycobacterium smegmatis 46.0 nM
Cytotoxicity against mouse RAW264.7 cells at 100 uM after 48 hrs by MTT assay Mus musculus 81.67 %
Inhibition of Mycobacterium smegmatis GyrB ATPase activity expressed in Escherichia coli BL21 (DE3) pLysS cells after 100 mins by inorganic phosphate release detection based malachite green reagent assay Mycobacterium smegmatis 180.0 nM
Inhibition of Mycobacterium tuberculosis GyrA/GyrB using relaxed pBR322 substrate incubated for 30 mins by ethidium bromide staining and electrophoresis based DNA supercoiling assay Mycobacterium tuberculosis 46.0 nM
Inhibition of Escherichia coli DNA Gyrase ATPase activity assessed as reduction in conversion of NADH to NAD+ using linear pBR322 substrate Escherichia coli 130.0 nM
Inhibition of Escherichia coli DNA gyrase using relaxed pNO1 plasmid and biotinylated oligonucelotide incubated for 30 mins by fluorescence based DNA super-coiling assay Escherichia coli 170.0 nM
Inhibition of Staphylococcus aureus DNA gyrase using relaxed pNO1 plasmid and biotinylated oligonucelotide incubated for 30 mins by fluorescence based DNA super-coiling assay Staphylococcus aureus 40.0 nM
Inhibition of Staphylococcus aureus DNA gyrase using relaxed pBR322 DNA as substrate after 3 hrs by DNA super-coiling assay Staphylococcus aureus 10.0 nM
Inhibition of Escherichia coli DNA gyrase using relaxed pBR322 DNA as substrate after 3 hrs by DNA super-coiling assay Escherichia coli 80.0 nM
Inhibition of Mycobacterium smegmatis DNA gyrase B expressed in Escherichia coli BL21 (DE3) pLysS cells assessed as reduction in ATPase activity incubated for 100 mins by inorganic phosphate detection assay Mycobacterium smegmatis 180.0 nM
Inhibition of DNA gyrase GyrA/GyrB in Mycobacterium tuberculosis incubated for 30 mins assessed as reduction in supercoiling of relaxed relaxed pBR322 DNA by ethidium bromide staining based agarose gel electrophoresis method Mycobacterium tuberculosis 46.0 nM
Cytotoxicity against mouse RAW264.7 cells at 50 uM after 48 hrs by MTT assay Mus musculus 19.3 %
Inhibition of Mycobacterium tuberculosis DNA gyrase assessed as inhibition of DNA supercoiling after 30 mins by electrophoresis Mycobacterium tuberculosis 46.0 nM
Inhibition of Escherichia coli DNA gyrase assessed as supercoiling of relaxed pNO1 plasmid after 30 mins by SybrGOLD staining based fluorescence assay Escherichia coli 168.0 nM
Inhibition of Staphylococcus aureus DNA gyrase assessed as supercoiling of relaxed pNO1 plasmid after 30 mins by SybrGOLD staining based fluorescence assay Staphylococcus aureus 41.0 nM
Binding affinity to Escherichia coli DNA gyrase B by surface plasmon resonance assay Escherichia coli 28.0 nM
Inhibition of Escherichia coli DNA gyrase assessed as supercoiling of relaxed pBR322 plasmid after 45 mins by ethidium bromide staining based agarose gel electrophoresis Escherichia coli 80.0 nM
Inhibition of Staphylococcus aureus DNA gyrase assessed as supercoiling of relaxed pBR322 plasmid after 45 mins by ethidium bromide staining based agarose gel electrophoresis Staphylococcus aureus 10.0 nM
Binding affinity to Escherichia coli DNA gyrase B Escherichia coli 19.0 nM
Inhibition of Escherichia coli DNA gyrase B ATP binding site assessed as reduction in supercoiling of relaxed pNO1 DNA after 30 mins in presence of biotinylated oligonucleotide by sybrGOLD staining based fluorescence assay Escherichia coli 170.0 nM
Inhibition of Staphylococcus aureus DNA gyrase B ATP binding site assessed as reduction in supercoiling of relaxed pNO1 DNA after 30 mins in presence of biotinylated oligonucleotide by sybrGOLD staining based fluorescence assay Staphylococcus aureus 40.0 nM
Inhibition of Mycobacterium smegmatis 155 6His-tagged DNA gyrase B catalytic domain ATPase activity expressed in Escherichia coli BL21 (DE3) pLysS cells assessed as inorganic phosphate release after 120 mins in presence of ATP by malachite green dye based assay Mycobacterium smegmatis str. MC2 155 46.0 nM
Inhibition of Mycobacterium tuberculosis DNA gyrase subunit A2B2 supercoiling activity using relaxed pBR322 as substrate after 30 mins in presence of ATP by agarose gel electrophoresis Mycobacterium tuberculosis 180.0 nM
Inhibition of Escherichia coli DNA gyrase supercoiling activity using relaxed pNO1 as substrate after 30 mins in presence of biotin-labeled oligonucleotide and ATP by SybrGOLD staining based fluorescence assay Escherichia coli 170.0 nM
Binding affinity to Escherichia coli H560 DNA gyrase B Escherichia coli 7.0 nM
Inhibition of Mycobacterium tuberculosis DNA gyrase assessed as inhibition of DNA supercoiling activity using relaxed pBR322 as substrate incubated for 30 mins by ethidium bromide staining based agarose gel electrophoresis Mycobacterium tuberculosis 46.0 nM
Inhibition of Escherichia coli H560 DNA gyrase B ATPase activity after 30 mins by ammonium molybdate/malachite green-based phosphate detection assay Escherichia coli 7.0 nM
Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell viability at 100 uM measured after 48 hrs by MTT assay relative to control Mus musculus 7.56 %
Inhibition of human DNA topoisomerase-2alpha ATPase activity at 400 uM using supercoiled pBR322 DNA as substrate after 1 hr by malachite green dye based assay relative to control Homo sapiens 55.9 %
Inhibition of human DNA topoisomerase-2alpha ATPase activity at 300 uM using supercoiled pBR322 DNA as substrate after 1 hr by malachite green dye based assay relative to control Homo sapiens 33.3 %
Inhibition of human DNA topoisomerase-2alpha ATPase activity at 200 uM using supercoiled pBR322 DNA as substrate after 1 hr by malachite green dye based assay relative to control Homo sapiens 22.7 %
Inhibition of Escherichia coli DNA gyrase A/DNA gyrase B supercoiling activity using relaxed pNO1 DNA as substrate after 30 mins in presence of biotin-labeled oligonucleotide by SybrGOLD staining based fluorescence assay Escherichia coli 170.0 nM
Inhibition of Staphylococcus aureus DNA gyrase A/DNA gyrase B supercoiling activity using relaxed pNO1 DNA as substrate after 30 mins in presence of biotin-labeled oligonucleotide by SybrGOLD staining based fluorescence assay Staphylococcus aureus 41.0 nM
Inhibition of Escherichia coli DNA gyrase supercoiling activity using relaxed pBR322 DNA as substrate after 45 mins by ethidium-bromide staining based agarose gel electrophoresis Escherichia coli 80.0 nM
Inhibition of Staphylococcus aureus DNA gyrase supercoiling activity using relaxed pBR322 DNA as substrate after 45 mins by ethidium-bromide staining based agarose gel electrophoresis Staphylococcus aureus 10.0 nM
Inhibition of bacterial DNA gyrase subunit B Bacteria 80.0 nM
Inhibition of Escherichia coli ATCC 25922 DNA gyrase B using relaxed pNO1 DNA as substrate incubated for 30 mins and measured by sybrGOLD staining based fluorescence assay Escherichia coli ATCC 25922 170.0 nM
Inhibition of Staphylococcus aureus DNA gyrase B using relaxed pNO1 DNA as substrate incubated for 30 mins and measured by sybrGOLD staining based fluorescence assay Staphylococcus aureus 41.0 nM
Inhibition of Escherichia coli DNA gyrase using relaxed pBR322 DNA as substrate after 3 hrs by agarose electrophoresis Escherichia coli 80.0 nM
Inhibition of Staphylococcus aureus DNA gyrase using relaxed pBR322 DNA as substrate after 3 hrs by agarose electrophoresis Staphylococcus aureus 10.0 nM
Inhibition of human DNA topoisomerase 2 ATPase activity at 200 mM by Topo-2 ATPase assay Homo sapiens 38.54 %
Inhibition of human DNA topoisomerase 2 ATPase activity at 400 mM by Topo-2 ATPase assay Homo sapiens 60.23 %
Inhibition of human DNA topoisomerase 2 ATPase activity at 300 mM by Topo-2 ATPase assay Homo sapiens 45.86 %
Inhibition of Mycobacterium tuberculosis DNA gyrase supercoiling activity using relaxed pBR322 as substrate at 10 uM after 30 mins in presence of ATP by ethidium bromide staining based agarose gel electrophoresis relative to control Mycobacterium tuberculosis 100.0 %
Inhibition of Staphylococcus aureus N-terminal His6-tagged DNA gyrase subunit GyrA/GyrB supercoiling activity expressed in Escherichia coli BL21 (DE3) using relaxed pBR322 DNA as substrate after 1 hr by agarose gel electrophoresis Staphylococcus aureus 125.0 nM
Inhibition of fluoroquinolone-resistant Staphylococcus aureus clinical isolate DNA gyrase subunit GyrB/GyrA S84L mutant supercoiling activity using relaxed pBR322 DNA as substrate after 1 hr by agarose gel electrophoresis Staphylococcus aureus 30.0 nM
Inhibition of fluoroquinolone-resistant Streptococcus pneumoniae A-70 clinical isolate DNA gyrase subunit GyrB/GyrA S81F mutant supercoiling activity using relaxed pBR322 DNA as substrate after 1 hr mins by agarose gel electrophoresis Streptococcus pneumoniae 500.0 nM
Inhibition of Haemophilus influenzae DNA gyrase subunit GyrA/GyrB supercoiling activity expressed in Escherichia coli BL21 (DE3) using relaxed pBR322 DNA as substrate after 1 hr mins by agarose gel electrophoresis Haemophilus influenzae 500.0 nM
Inhibition of Escherichia coli C-terminal His6-tagged DNA gyrase B expressed in Escherichia coli BL21(DE3) preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method Escherichia coli 170.0 nM
Inhibition of Staphylococcus aureus DNA gyrase B preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method Staphylococcus aureus 41.0 nM
Cytotoxicity against human HepG2 cells after 24 hrs by CellTiter-Glo assay Homo sapiens 32.0 ug.mL-1
Inhibition of Mycobacterium tuberculosis DNA gyrase activity Mycobacterium tuberculosis 85.0 nM
Inhibition of Mycobacterium tuberculosis DNA gyrase ATPase activity Mycobacterium tuberculosis 20.0 nM
Inhibition of Escherichia coli DNA gyrase assessed as inhibition of pUC19 supercoiling after 30 mins by agarose gel electrophoresis Escherichia coli 144.0 nM
Inhibition of Escherichia coli DNA gyrase ATPase activity using DNA as substrate after 60 mins Escherichia coli 245.0 nM
Inhibition of Escherichia coli DNA topoisomerase-2 mediated DNA relaxation using supercoiled pBR322 plasmid DNA as substrate after 60 mins by ethidium bromide staining based agarose gel electrophoresis Escherichia coli 0.5 ug.mL-1
Inhibition of Escherichia coli DNA topoisomerase-4 mediated decatenation using kinetoplast DNA as substrate after 30 mins agarose gel electrophoresis method Escherichia coli 4.0 ug.mL-1
Inhibition of Staphylococcus aureus DNA gyrase supercoiling activity using relaxed pHOT-1 DNA as substrate after 60 mins by ethidium bromide-based gel electrophoresis Staphylococcus aureus 40.0 nM
Inhibition of NS2B-NS3 protease in Zika virus Puerto Rico/PRVABC5 infected in African green monkey Vero cells assessed as antiviral activity measured 48 hrs post infection by RT-PCR method Zika virus 15.21 ug.mL-1
Inhibition of NS2B-NS3 protease in Zika virus Puerto Rico/PRVABC5 infected in human Huh7 cells assessed as antiviral activity measured 48 hrs post infection by RT-PCR method Zika virus 15.21 ug.mL-1
Inhibition of NS2B-NS3 protease in Zika virus Puerto Rico/PRVABC5 assessed as antiviral activity measured 48 hrs post infection by plaque reduction assay Zika virus 15.21 ug.mL-1
Inhibition of Escherichia coli DNA gyrase using relaxed pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay Escherichia coli 170.0 nM
Inhibition of Staphylococcus aureus DNA gyrase using relaxed pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay Staphylococcus aureus 41.0 nM
SARS-CoV-2 3CL-Pro protease inhibition percentage at 20µM by FRET kind of response from peptide substrate Severe acute respiratory syndrome coronavirus 2 6.53 %
Antiviral activity determined as inhibition of SARS-CoV-2 induced cytotoxicity of VERO-6 cells at 10 uM after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging Chlorocebus sabaeus -0.03 % Antiviral activity determined as inhibition of SARS-CoV-2 induced cytotoxicity of VERO-6 cells at 10 uM after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging Chlorocebus sabaeus -0.03 %
Inhibition of Staphylococcus aureus DNA gyrase using biotinylated oligonucleotide as substrate incubated for 30 mins in presence of ATP by fluorescence-based microplate reader assay Staphylococcus aureus 41.0 nM
Inhibition of Escherichia coli DNA gyrase using biotinylated oligonucleotide as substrate incubated for 30 mins in presence of ATP by fluorescence-based microplate reader assay Escherichia coli 170.0 nM
Inhibition of Escherichia coli DNA gyrase using relaxed pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay Escherichia coli 168.0 nM
Inhibition of Staphylococcus aureus DNA gyrase using relaxed pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay Staphylococcus aureus 41.0 nM
Inhibition of Staphylococcus aureus Gyrase B using linear pBR322 DNA as substrate incubated for 30 mins in presence of ATP by ADP-Glo assay Staphylococcus aureus 15.0 nM
Inhibition of Escherichia coli DNA gyrase by measuring supercoiling activity incubated for 60 mins by fluorescence based assay Escherichia coli 23.88 nM
Inhibition of Escherichia coli topoisomerase IV by measuring relaxation activity incubated for 30 mins by fluorescence based assay Escherichia coli 820.4 nM
Inhibition of Escherichia coli DNA gyrase assessed as reduction in relaxation of supercoiled pNO1 DNA measured after 30 mins by fluorescence assay Escherichia coli 170.0 nM
Inhibition of Staphylococcus aureus DNA gyrase assessed reduction in relaxation of supercoiled pNO1 DNA measured after 30 mins by fluorescence assay Staphylococcus aureus 34.0 nM

Cross References

Resources Reference
ChEBI 28368
ChEMBL CHEMBL36506
DrugBank DB01051
DrugCentral 1974
FDA SRS 17EC19951N
Human Metabolome Database HMDB0015185
KEGG C05080
PDB NOV
PubChem 54675769
SureChEMBL SCHEMBL24805
ZINC ZINC000014879999
CONTENTS