|
Fungicidal activity against Pestalotiopsis microspora isolates infected Chinese hickory orchards by conidium germination test
|
Pestalotiopsis microspora
|
0.13
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R3 to EC50 for wild type Pestalotiopsis microspora isolate CA-8
|
Pestalotiopsis microspora
|
106.0
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R2 to EC50 for wild type Pestalotiopsis microspora isolate CA-8
|
Pestalotiopsis microspora
|
240.0
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R1 to EC50 for wild type Pestalotiopsis microspora isolate CA-8
|
Pestalotiopsis microspora
|
204.0
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate LA1R2 to EC50 for wild type Pestalotiopsis microspora isolate LA-1
|
Pestalotiopsis microspora
|
406.0
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Resistance factor, ratio of EC50 for pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate LA1R1 to EC50 for wild type Pestalotiopsis microspora isolate LA-1
|
Pestalotiopsis microspora
|
179.0
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R3 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
4.25
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R2 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
9.59
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate CA8R1 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
8.17
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against wild type Pestalotiopsis microspora isolate CA-8 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
0.04
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate LA1R2 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
12.19
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against pyraclostrobin-resistant mutant Pestalotiopsis microspora isolate LA1R1 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
5.67
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against wild type Pestalotiopsis microspora isolate LA-1 assessed as conidium germination inhibition at 25 degC
|
Pestalotiopsis microspora
|
0.03
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against Pestalotiopsis microspora infected in Chinese hickory orchards assessed as conidium germination inhibition at 25 degC after 24 hr
|
Pestalotiopsis microspora
|
0.08
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Fungicidal activity against Pestalotiopsis microspora infected Chinese hickory orchards assessed as mycelial growth inhibition at 25 degC after 5 days
|
Pestalotiopsis microspora
|
0.92
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 42
First Page : 256
Last Page : 259
|
Curative effect against Rhizoctonia solani AG-1 IA infected soybean plant at 100 g a.i/ha applied as spray at V5 to V6 growth stage post inoculation measured after 5 to 10 days under greenhouse condition relative to control
|
Rhizoctonia solani AG-1 IA
|
None
|
|
Journal : Crop Protection
Title : Effect of doses of fungicides and plant resistance activators on the control of Rhizoctonia foliar blight of soybean, and on Rhizoctonia solani AG1-IA in vitro development
Year : 2006
Volume : 25
Issue : 8
First Page : 848
Last Page : 854
Authors : Meyer MC, Bueno CJ, Souza NLde, Yorinori JT.
Abstract : Rhizoctonia foliar blight (RFB) of soybean [Glycine max (L.) Merrill] occurs in many tropical and subtropical regions, causing yield reductions of up to 70% and in Brazil, up to 60%. The disease is caused by Rhizoctonia solani AG1-IA and AG1-IB, and by AG2-3 in Japan. RFB occurs in the North, Northeast and Mid-west regions of Brazil. Chemical control remains the only effective method of controlling RFB, but its efficiency depends upon environmental conditions. In this study, 18 fungicides, salicylic acid (SA) and acibenzolar-s-methyl (ASM) were evaluated on R. solani AG1-IA in vitro, by mycelial growth rating and estimating effective concentration for 50% (EC 50) and 90% (EC 90) inhibition of mycelial growth, and in vivo by reduction of disease severity on soybean plants in greenhouse conditions. Mycelial growth was strongly inhibited by the fungicides pyraclostrobin+boscalid and fludioxonil. Preventive fungicide applications were the most effective. Strobilurins were more efficient both in preventive and curative applications. Best results with plant resistance activators were obtained with SA (2.5 mM) sprayed at 20 d before inoculation and with ASM (12.5 mg a.i. l-1) 10 d before inoculation.
|
Protective effect against Rhizoctonia solani AG-1 IA infected soybean plant at 100 g a.i/ha applied as spray at V5 to V6 growth stage prior to inoculation measured after 5 to 10 days under greenhouse condition relative to control
|
Rhizoctonia solani AG-1 IA
|
None
|
|
Journal : Crop Protection
Title : Effect of doses of fungicides and plant resistance activators on the control of Rhizoctonia foliar blight of soybean, and on Rhizoctonia solani AG1-IA in vitro development
Year : 2006
Volume : 25
Issue : 8
First Page : 848
Last Page : 854
Authors : Meyer MC, Bueno CJ, Souza NLde, Yorinori JT.
Abstract : Rhizoctonia foliar blight (RFB) of soybean [Glycine max (L.) Merrill] occurs in many tropical and subtropical regions, causing yield reductions of up to 70% and in Brazil, up to 60%. The disease is caused by Rhizoctonia solani AG1-IA and AG1-IB, and by AG2-3 in Japan. RFB occurs in the North, Northeast and Mid-west regions of Brazil. Chemical control remains the only effective method of controlling RFB, but its efficiency depends upon environmental conditions. In this study, 18 fungicides, salicylic acid (SA) and acibenzolar-s-methyl (ASM) were evaluated on R. solani AG1-IA in vitro, by mycelial growth rating and estimating effective concentration for 50% (EC 50) and 90% (EC 90) inhibition of mycelial growth, and in vivo by reduction of disease severity on soybean plants in greenhouse conditions. Mycelial growth was strongly inhibited by the fungicides pyraclostrobin+boscalid and fludioxonil. Preventive fungicide applications were the most effective. Strobilurins were more efficient both in preventive and curative applications. Best results with plant resistance activators were obtained with SA (2.5 mM) sprayed at 20 d before inoculation and with ASM (12.5 mg a.i. l-1) 10 d before inoculation.
|
Fungicidal activity against Rhizoctonia solani AG-1 IA assessed as inhibition of mycelial radial growth
|
Rhizoctonia solani AG-1 IA
|
4.68
mg/L
|
|
Fungicidal activity against Rhizoctonia solani AG-1 IA assessed as inhibition of mycelial radial growth
|
Rhizoctonia solani AG-1 IA
|
0.094
ug.mL-1
|
|
Journal : Crop Protection
Title : Effect of doses of fungicides and plant resistance activators on the control of Rhizoctonia foliar blight of soybean, and on Rhizoctonia solani AG1-IA in vitro development
Year : 2006
Volume : 25
Issue : 8
First Page : 848
Last Page : 854
Authors : Meyer MC, Bueno CJ, Souza NLde, Yorinori JT.
Abstract : Rhizoctonia foliar blight (RFB) of soybean [Glycine max (L.) Merrill] occurs in many tropical and subtropical regions, causing yield reductions of up to 70% and in Brazil, up to 60%. The disease is caused by Rhizoctonia solani AG1-IA and AG1-IB, and by AG2-3 in Japan. RFB occurs in the North, Northeast and Mid-west regions of Brazil. Chemical control remains the only effective method of controlling RFB, but its efficiency depends upon environmental conditions. In this study, 18 fungicides, salicylic acid (SA) and acibenzolar-s-methyl (ASM) were evaluated on R. solani AG1-IA in vitro, by mycelial growth rating and estimating effective concentration for 50% (EC 50) and 90% (EC 90) inhibition of mycelial growth, and in vivo by reduction of disease severity on soybean plants in greenhouse conditions. Mycelial growth was strongly inhibited by the fungicides pyraclostrobin+boscalid and fludioxonil. Preventive fungicide applications were the most effective. Strobilurins were more efficient both in preventive and curative applications. Best results with plant resistance activators were obtained with SA (2.5 mM) sprayed at 20 d before inoculation and with ASM (12.5 mg a.i. l-1) 10 d before inoculation.
|
Fungicidal activity against Rhizoctonia solani AG-1 IA assessed as mycelial radial growth at 100 mg/l (Rvb = 40 mm)
|
Rhizoctonia solani AG-1 IA
|
0.0
mm
|
|
Journal : Crop Protection
Title : Effect of doses of fungicides and plant resistance activators on the control of Rhizoctonia foliar blight of soybean, and on Rhizoctonia solani AG1-IA in vitro development
Year : 2006
Volume : 25
Issue : 8
First Page : 848
Last Page : 854
Authors : Meyer MC, Bueno CJ, Souza NLde, Yorinori JT.
Abstract : Rhizoctonia foliar blight (RFB) of soybean [Glycine max (L.) Merrill] occurs in many tropical and subtropical regions, causing yield reductions of up to 70% and in Brazil, up to 60%. The disease is caused by Rhizoctonia solani AG1-IA and AG1-IB, and by AG2-3 in Japan. RFB occurs in the North, Northeast and Mid-west regions of Brazil. Chemical control remains the only effective method of controlling RFB, but its efficiency depends upon environmental conditions. In this study, 18 fungicides, salicylic acid (SA) and acibenzolar-s-methyl (ASM) were evaluated on R. solani AG1-IA in vitro, by mycelial growth rating and estimating effective concentration for 50% (EC 50) and 90% (EC 90) inhibition of mycelial growth, and in vivo by reduction of disease severity on soybean plants in greenhouse conditions. Mycelial growth was strongly inhibited by the fungicides pyraclostrobin+boscalid and fludioxonil. Preventive fungicide applications were the most effective. Strobilurins were more efficient both in preventive and curative applications. Best results with plant resistance activators were obtained with SA (2.5 mM) sprayed at 20 d before inoculation and with ASM (12.5 mg a.i. l-1) 10 d before inoculation.
|
Fungicidal activity against Rhizoctonia solani AG-1 IA assessed as mycelial radial growth at 1 mg/l (Rvb = 40 mm)
|
Rhizoctonia solani AG-1 IA
|
8.1
mm
|
|
Journal : Crop Protection
Title : Effect of doses of fungicides and plant resistance activators on the control of Rhizoctonia foliar blight of soybean, and on Rhizoctonia solani AG1-IA in vitro development
Year : 2006
Volume : 25
Issue : 8
First Page : 848
Last Page : 854
Authors : Meyer MC, Bueno CJ, Souza NLde, Yorinori JT.
Abstract : Rhizoctonia foliar blight (RFB) of soybean [Glycine max (L.) Merrill] occurs in many tropical and subtropical regions, causing yield reductions of up to 70% and in Brazil, up to 60%. The disease is caused by Rhizoctonia solani AG1-IA and AG1-IB, and by AG2-3 in Japan. RFB occurs in the North, Northeast and Mid-west regions of Brazil. Chemical control remains the only effective method of controlling RFB, but its efficiency depends upon environmental conditions. In this study, 18 fungicides, salicylic acid (SA) and acibenzolar-s-methyl (ASM) were evaluated on R. solani AG1-IA in vitro, by mycelial growth rating and estimating effective concentration for 50% (EC 50) and 90% (EC 90) inhibition of mycelial growth, and in vivo by reduction of disease severity on soybean plants in greenhouse conditions. Mycelial growth was strongly inhibited by the fungicides pyraclostrobin+boscalid and fludioxonil. Preventive fungicide applications were the most effective. Strobilurins were more efficient both in preventive and curative applications. Best results with plant resistance activators were obtained with SA (2.5 mM) sprayed at 20 d before inoculation and with ASM (12.5 mg a.i. l-1) 10 d before inoculation.
|
Fungicidal activity against Rhizoctonia solani AG-1 IA assessed as mycelial radial growth at 10 mg/l (Rvb = 40 mm)
|
Rhizoctonia solani AG-1 IA
|
5.4
mm
|
|
Journal : Crop Protection
Title : Effect of doses of fungicides and plant resistance activators on the control of Rhizoctonia foliar blight of soybean, and on Rhizoctonia solani AG1-IA in vitro development
Year : 2006
Volume : 25
Issue : 8
First Page : 848
Last Page : 854
Authors : Meyer MC, Bueno CJ, Souza NLde, Yorinori JT.
Abstract : Rhizoctonia foliar blight (RFB) of soybean [Glycine max (L.) Merrill] occurs in many tropical and subtropical regions, causing yield reductions of up to 70% and in Brazil, up to 60%. The disease is caused by Rhizoctonia solani AG1-IA and AG1-IB, and by AG2-3 in Japan. RFB occurs in the North, Northeast and Mid-west regions of Brazil. Chemical control remains the only effective method of controlling RFB, but its efficiency depends upon environmental conditions. In this study, 18 fungicides, salicylic acid (SA) and acibenzolar-s-methyl (ASM) were evaluated on R. solani AG1-IA in vitro, by mycelial growth rating and estimating effective concentration for 50% (EC 50) and 90% (EC 90) inhibition of mycelial growth, and in vivo by reduction of disease severity on soybean plants in greenhouse conditions. Mycelial growth was strongly inhibited by the fungicides pyraclostrobin+boscalid and fludioxonil. Preventive fungicide applications were the most effective. Strobilurins were more efficient both in preventive and curative applications. Best results with plant resistance activators were obtained with SA (2.5 mM) sprayed at 20 d before inoculation and with ASM (12.5 mg a.i. l-1) 10 d before inoculation.
|
Fungicidal activity against Rhizoctonia solani AG-1 IA assessed as mycelial radial growth at 0.1 mg/l (Rvb = 40 mm)
|
Rhizoctonia solani AG-1 IA
|
17.8
mm
|
|
Journal : Crop Protection
Title : Effect of doses of fungicides and plant resistance activators on the control of Rhizoctonia foliar blight of soybean, and on Rhizoctonia solani AG1-IA in vitro development
Year : 2006
Volume : 25
Issue : 8
First Page : 848
Last Page : 854
Authors : Meyer MC, Bueno CJ, Souza NLde, Yorinori JT.
Abstract : Rhizoctonia foliar blight (RFB) of soybean [Glycine max (L.) Merrill] occurs in many tropical and subtropical regions, causing yield reductions of up to 70% and in Brazil, up to 60%. The disease is caused by Rhizoctonia solani AG1-IA and AG1-IB, and by AG2-3 in Japan. RFB occurs in the North, Northeast and Mid-west regions of Brazil. Chemical control remains the only effective method of controlling RFB, but its efficiency depends upon environmental conditions. In this study, 18 fungicides, salicylic acid (SA) and acibenzolar-s-methyl (ASM) were evaluated on R. solani AG1-IA in vitro, by mycelial growth rating and estimating effective concentration for 50% (EC 50) and 90% (EC 90) inhibition of mycelial growth, and in vivo by reduction of disease severity on soybean plants in greenhouse conditions. Mycelial growth was strongly inhibited by the fungicides pyraclostrobin+boscalid and fludioxonil. Preventive fungicide applications were the most effective. Strobilurins were more efficient both in preventive and curative applications. Best results with plant resistance activators were obtained with SA (2.5 mM) sprayed at 20 d before inoculation and with ASM (12.5 mg a.i. l-1) 10 d before inoculation.
|
Fungicidal activity against Monilinia laxa isolate MLX1 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
29.41
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLP4 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
47.32
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLK12 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
37.5
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLK1 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
50.05
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLC2 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
34.48
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLX5 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
51.15
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLP2 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
50.0
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLBO5 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
49.62
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLBO2 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
40.65
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLA1 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
36.82
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Fungicidal activity against Monilinia laxa isolate MLG1 assessed as mycelial growth at 0.05 ug/ml at 25 degC after 4 days
|
Monilinia laxa
|
25.0
%
|
|
Journal : Crop Protection
Title : PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece
Year : 2012
Volume : 39
First Page : 11
Last Page : 17
Authors : Malandrakis AA, Markoglou AN, Ziogas BN.
Abstract : Sensitivity to benzimidazoles in isolates of the brown rot pathogen (Monilinia laxa) collected from stone fruit in central and northern Greece was evaluated and the molecular basis for resistance was investigated. M. laxa isolates were classified as benzimidazole – sensitive (S) or highly resistant (HR) based on their sensitivity profiles to carbendazim. Thirty seven percent of the isolates belonged to the HR phenotype, carried no apparent fitness penalties and exhibited resistance factor values (based on EC50 values) greater than 500. Highly resistant isolates were also less sensitive to the benzimidazoles benomyl and thiophanate-methyl but more sensitive to the N-phenylcarbamate diethofencarb and the benzamide zoxamide compared to isolates belonging to the S phenotype. Fungitoxicity tests with fungicides belonging to other chemical classes revealed no cross resistance relationships between benzimidazoles and the dicarboximide iprodione, the phenylpyrrole fludioxonil, the hydroxyanilide fenhexamid, the carboxamide boscalid, the triazole tebuconazole and the strobilurin-type fungicide pyraclostrobin, indicating that a target site modification is probably responsible for the resistant phenotypes observed. Comparison of the β-tubulin gene DNA sequences between resistant and sensitive isolates revealed a point mutation resulting from the E198A substitution of the corresponding protein in all HR isolates tested. An Eco31I restriction site in the β-tubulin gene, which was destroyed in HR M. laxa isolates, allowed the development of a PCR-RFLP diagnostic for the detection of the E198A resistance mutation.
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 115-2-3 assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0124
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 4b assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0038
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 90 P7d assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0033
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 120-2-2 assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0081
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 90 DL-22 assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0118
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 1A assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0099
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 119-2-3 assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0038
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 148-1-1 assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0051
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 86-4 assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0034
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 145-2-4 assessed as inhibition of conidia germination after 6 hr incubation
|
Pyrenophora tritici-repentis
|
0.0038
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 115-2-3 assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.0023
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 4b assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.0016
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 90 P7d assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.0016
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 120-2-2 assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.0021
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 90 DL-22 assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.0016
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 1A assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.0022
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 119-2-3 assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.002
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 148-1-1 assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.0023
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 86-4 assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.0016
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against Pyrenophora tritici-repentis isolate Ptr 92 145-2-4 assessed as inhibition of conidia germination after 6 hr incubation in presence of SHAM
|
Pyrenophora tritici-repentis
|
0.0015
ug.mL-1
|
|
Journal : Crop Protection
Year : 2012
Volume : 34
First Page : 37
Last Page : 41
|
Antifungal activity against logarithmic phase of Pythium ultimum Trow ATCC 58812 assessed as growth inhibition measured at 5 days post-drug treatment
|
Pythium ultimum
|
10000.0
nM
|
|
Journal : J Agric Food Chem
Year : 2007
Volume : 55
Issue : 25
First Page : 10331
Last Page : 10338
|
Antifungal activity against logarithmic phase of Pythium ultimum ATCC 58812 assessed as growth inhibition at 100 ug/ml measured at 5 days post-drug treatment
|
Pythium ultimum
|
100.0
%
|
|
Journal : J Agric Food Chem
Year : 2007
Volume : 55
Issue : 25
First Page : 10331
Last Page : 10338
|
Antifungal activity against logarithmic phase of Pythium ultimum ATCC 58812 assessed as growth inhibition at 50 ug/ml measured at 5 days post-drug treatment
|
Pythium ultimum
|
100.0
%
|
|
Journal : J Agric Food Chem
Year : 2007
Volume : 55
Issue : 25
First Page : 10331
Last Page : 10338
|
Antifungal activity against logarithmic phase of Pythium ultimum ATCC 58812 assessed as growth inhibition at 10 ug/ml measured at 5 days post-drug treatment
|
Pythium ultimum
|
100.0
%
|
|
Journal : J Agric Food Chem
Year : 2007
Volume : 55
Issue : 25
First Page : 10331
Last Page : 10338
|
Inhibition of Sus scrofa (pig) heart cytochrome bc1 complex using DBH2 as substrate by spectrophotometric analysis
|
Sus scrofa
|
3.3
nM
|
|
Journal : J Am Chem Soc
Year : 2010
Volume : 132
Issue : 1
First Page : 185
Last Page : 194
|
Antifungal activity against Monilinia fructicola isolated from peach fields assessed as mycelial growth inhibition
|
Monilinia fructicola
|
0.04
ppm
|
|
Journal : Crop Protection
Year : 2011
Volume : 30
Issue : 11
First Page : 1461
Last Page : 1468
|
Antifungal activity against citrus greasy spot disease caused by Zasmidium citri assessed as inhibition of mycelial growth on potato dextrose agar
|
Zasmidium citri
|
0.03
ppm
|
|
Journal : Crop Protection
Year : 2011
Volume : 30
Issue : 11
First Page : 1461
Last Page : 1468
|
Antifungal activity against circular leaf spot causing Mycosphaerella nawae MY1 assessed as inhibition of mycelial growth on potato dextrose agar
|
Mycosphaerella nawae
|
1.6
ug.mL-1
|
|
Journal : Crop Protection
Year : 2011
Volume : 30
Issue : 11
First Page : 1461
Last Page : 1468
|
Antifungal activity against circular leaf spot causing Mycosphaerella nawae MY5 assessed as inhibition of mycelial growth on potato dextrose agar
|
Mycosphaerella nawae
|
1.9
ug.mL-1
|
|
Journal : Crop Protection
Year : 2011
Volume : 30
Issue : 11
First Page : 1461
Last Page : 1468
|
Antifungal activity against Fusarium equiseti (CE181) after 48 to 72 hr
|
Fusarium equiseti
|
12.5
ug.mL-1
|
|
Journal : J Agric Food Chem
Title : Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.
Year : 2004
Volume : 52
Issue : 11
First Page : 3297
Last Page : 3300
Authors : Svetaz L, Tapia A, López SN, Furlán RL, Petenatti E, Pioli R, Schmeda-Hirschmann G, Zacchino SA.
Abstract : The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL
|
Antifungal activity against Diaporthe longicolla CE117 after 48 to 72 hr
|
Diaporthe longicolla
|
0.01
ug.mL-1
|
|
Journal : J Agric Food Chem
Title : Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.
Year : 2004
Volume : 52
Issue : 11
First Page : 3297
Last Page : 3300
Authors : Svetaz L, Tapia A, López SN, Furlán RL, Petenatti E, Pioli R, Schmeda-Hirschmann G, Zacchino SA.
Abstract : The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL
|
Antifungal activity against Fusarium graminearum isolate CE169 after 48 to 72 hr
|
Fusarium graminearum
|
20.0
ug.mL-1
|
|
Journal : J Agric Food Chem
Title : Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.
Year : 2004
Volume : 52
Issue : 11
First Page : 3297
Last Page : 3300
Authors : Svetaz L, Tapia A, López SN, Furlán RL, Petenatti E, Pioli R, Schmeda-Hirschmann G, Zacchino SA.
Abstract : The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL
|
Antifungal activity against Fusarium graminearum isolate CE171 after 48 to 72 hr
|
Fusarium graminearum
|
0.05
ug.mL-1
|
|
Journal : J Agric Food Chem
Title : Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.
Year : 2004
Volume : 52
Issue : 11
First Page : 3297
Last Page : 3300
Authors : Svetaz L, Tapia A, López SN, Furlán RL, Petenatti E, Pioli R, Schmeda-Hirschmann G, Zacchino SA.
Abstract : The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL
|
Antifungal activity against Fusarium graminearum isolate CE135 after 48 to 72 hr
|
Fusarium graminearum
|
1.0
ug.mL-1
|
|
Journal : J Agric Food Chem
Title : Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.
Year : 2004
Volume : 52
Issue : 11
First Page : 3297
Last Page : 3300
Authors : Svetaz L, Tapia A, López SN, Furlán RL, Petenatti E, Pioli R, Schmeda-Hirschmann G, Zacchino SA.
Abstract : The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL
|
Antifungal activity against Fusarium graminearum isolate CE170 after 48 to 72 hr
|
Fusarium graminearum
|
0.05
ug.mL-1
|
|
Journal : J Agric Food Chem
Title : Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.
Year : 2004
Volume : 52
Issue : 11
First Page : 3297
Last Page : 3300
Authors : Svetaz L, Tapia A, López SN, Furlán RL, Petenatti E, Pioli R, Schmeda-Hirschmann G, Zacchino SA.
Abstract : The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL
|
Antifungal activity against Macrophomina phaseolina isolate CE173 after 48 to 72 hr
|
Macrophomina phaseolina
|
2.5
ug.mL-1
|
|
Journal : J Agric Food Chem
Title : Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.
Year : 2004
Volume : 52
Issue : 11
First Page : 3297
Last Page : 3300
Authors : Svetaz L, Tapia A, López SN, Furlán RL, Petenatti E, Pioli R, Schmeda-Hirschmann G, Zacchino SA.
Abstract : The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL
|
Antifungal activity against Colletotrichum truncatum CE175 after 48 to 72 hr
|
Colletotrichum truncatum
|
0.04
ug.mL-1
|
|
Journal : J Agric Food Chem
Title : Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.
Year : 2004
Volume : 52
Issue : 11
First Page : 3297
Last Page : 3300
Authors : Svetaz L, Tapia A, López SN, Furlán RL, Petenatti E, Pioli R, Schmeda-Hirschmann G, Zacchino SA.
Abstract : The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL
|
Antifungal activity against Alternaria alternata (CE172) after 48 to 72 hr
|
Alternaria alternata
|
0.4
ug.mL-1
|
|
Journal : J Agric Food Chem
Title : Antifungal chalcones and new caffeic acid esters from Zuccagnia punctata acting against soybean infecting fungi.
Year : 2004
Volume : 52
Issue : 11
First Page : 3297
Last Page : 3300
Authors : Svetaz L, Tapia A, López SN, Furlán RL, Petenatti E, Pioli R, Schmeda-Hirschmann G, Zacchino SA.
Abstract : The crude methanolic extract of Zuccagnia punctata was active toward the fungal pathogens of soybean Phomopsis longicolla and Colletotrichum truncatum. Assay guided fractionation led to the isolation of two chalcones, one flavanone and a new caffeoyl ester derivative as the compounds responsible for the antifungal activity. Another new caffeoyl ester derivative was isolated from the antifungal chloroform extract but proved to be inactive against the soybean infecting fungi up to 50 microg/mL
|
Fungitoxicity against highly fenhexamid resistant Monilinia laxa MF-2 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.5
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Fungitoxicity against highly fenhexamid resistant Monilinia laxa MF-3 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.4
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Fungitoxicity against moderately fenhexamid resistant Monilinia laxa MF-7 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.4
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Fungitoxicity against highly fenhexamid resistant Monilinia laxa MF-5 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.4
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Fungitoxicity against moderately fenhexamid resistant Monilinia laxa MF-4 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.3
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Fungitoxicity against moderately fenhexamid resistant Monilinia laxa MF-22 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.2
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Fungitoxicity against moderately fenhexamid resistant Monilinia laxa MF-8 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.4
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Fungitoxicity against moderately fenhexamid resistant Monilinia laxa MF-6 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.1
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Fungitoxicity against moderately fenhexamid resistant Monilinia laxa MF-1 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.2
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Antifungal activity against Cercospora beticola isolate 11-1046-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
26.21
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1046-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
41.71
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1045-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
16.24
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1044-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
5.23
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1044-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
14.27
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 98-9-M expressing cytb with glycine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.001
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 98-10-M expressing cytb with glycine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.001
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 98-12-M expressing cytb with glycine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.001
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 98-13-M expressing cytb with glycine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.006
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 98-19-M expressing cytb with glycine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.003
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 98-101-5-M expressing cytb with glycine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.001
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 98-150-M expressing cytb with glycine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.001
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 99-233-3-M expressing cytb with glycine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.001
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 99-521-29-M expressing cytb with glycine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.001
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1003-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
0.915
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1004-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
5.35
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1005-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
46.02
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1005-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
7.87
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1006-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
9.54
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1007-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
4.78
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1007-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
16.11
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1008-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
4.82
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1009-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
47.42
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1014-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
5.03
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1015-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
72.23
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1015-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
2.1
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1016-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
51.72
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1022-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
29.68
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1022-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
6.37
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1026-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
7.15
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1026-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
7.56
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Fungitoxicity against wild type Monilinia laxa K2 assessed as reduction in mycelial growth measured at 25 degC after 4 days
|
Monilinia laxa
|
0.3
ug.mL-1
|
|
Journal : Crop Protection
Title : Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants
Year : 2013
Volume : 46
First Page : 13
Last Page : 17
Authors : Malandrakis A, Anastasios Markoglou, George Karaoglanidis, Nikolaos Koukiasas, Thomas Veloukas.
Abstract : Fenhexamid is a hydroxyanilide fungicide with excellent performance against Botrytis cinerea but also effective against Monilinia spp. which cause brown rot disease in apple and stone fruit. A total of 75 Monilinia laxa field isolates were utilized to determine baseline sensitivity while a number of fenhexamid-resistant laboratory mutants were used to evaluate the resistance risk associated with the longevity of the effectiveness of fenhexamid. Fenhexamid was found to be highly effective against all field isolates. EC50 values ranged from 0.02 to 1 μg mL−1 and were distributed unimodaly around an average of 0.1 μg mL−1. M. laxa laboratory strains with moderate and high resistance levels to fenhexamid were isolated after UV mutagenesis. All fenhexamid-resistant strains showed parental sensitivity to carbendazim, iprodione, fludioxonil, pyraclostrobin, flusilazole and prochloraz. Interestingly, some of the mutant strains were also resistant to tridemorph, fenpropimorph and spiroxamine. Studies on fitness parameters of fenhexamid-resistant strains revealed a fitness cost on sporulation and pathogenicity but not on mycelial growth. These results suggest that fenhexamid should be a good alternative site-specific fungicide for the control of brown rot disease caused by M. laxa. However, appropriate anti-resistance strategies should be considered to ensure the successful commercial use of fenhexamid in the long run.
|
Antifungal activity against Cercospora beticola isolate 11-1027-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
25.95
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1027-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
4.12
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1028-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
8.79
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1028-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
4.53
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1035-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
31.02
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1036-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
29.68
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1036-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
5.55
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1037-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
40.86
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1037-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
5.92
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1038-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
39.88
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1038-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
3.24
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1039-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
20.42
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1040-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
12.01
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1040-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
22.33
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1041-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
4.55
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1042-1 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
31.39
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1042-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
32.63
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Cercospora beticola isolate 11-1043-2 expressing cytb with alanine at codon 143 assessed as reduction in spore germination
|
Cercospora beticola
|
16.93
ug.mL-1
|
|
Journal : Pest Manag Sci
Year : 2013
Volume : 69
Issue : 1
First Page : 35
Last Page : 39
|
Antifungal activity against Botryotinia fuckeliana inoculated in strawberry fruit
|
Botryotinia fuckeliana
|
0.01
ug.mL-1
|
|
Journal : Pest Manag Sci
Title : Biological activity of the succinate dehydrogenase inhibitor fluopyram against Botrytis cinerea and fungal baseline sensitivity.
Year : 2012
Volume : 68
Issue : 6
First Page : 858
Last Page : 864
Authors : Veloukas T, Karaoglanidis GS.
Abstract : BACKGROUND: Succinate dehydrogenase inhibitors (SDHIs) constitute a fungicide class with increasing relevance in crop protection. These fungicides could play a crucial role in successful management of grey mould disease. In the present study the effect of fluopyram, a novel SDHI fungicide, on several developmental stages of Botrytis cinerea was determined in vitro, and the protective and curative activity against the pathogen was determined on strawberry fruit. Furthermore, fungal baseline sensitivity was determined in a set of 192 pathogen isolates. RESULTS: Inhibition of germ tube elongation was found to be the most sensitive growth stage affected by fluopyram, while mycelial growth was found to be the least sensitive growth stage. Fluopyram provided excellent protective activity against B. cinerea when applied at 100 µg mL(-1) 96, 48 or 24 h before the artificial inoculation of the strawberry fruit. Similarly, fluopyram showed a high curative activity when it was applied at 100 µg mL(-1) 24 h post-inoculation, but, when applications were conducted 48 or 96 h post-inoculation, disease control efficacy was modest or low. The measurement of baseline sensitivity showed that it was unimodal in all the populations tested. The individual EC(50) values for fluopyram ranged from 0.03 to 0.29 µg mL(-1). In addition, no correlation was found between sensitivity to fluopyram and sensitivity to other fungicides, including cyprodinil, fenhexamid, fludioxonil, iprodione, boscalid and pyraclostrobin. CONCLUSIONS: The obtained biological activity, baseline sensitivity and cross-resistance relationship data suggest that fluopyram could play a key role in grey mould management in the near future and encourage its introduction into spray programmes.
