|
Inhibition of COX2 at 100 uM by scintillation proximity assay
|
None
|
30.0
%
|
|
|
Inhibition of Na+/K+ ATPase
|
None
|
150.0
nM
|
|
|
Inhibition of HIV1 RT
|
Human immunodeficiency virus 1
|
200.0
ug.mL-1
|
|
|
Inhibition of Na+K+ATpase from pig cortex
|
Sus scrofa
|
600.0
nM
|
|
|
Inhibition of pig cerebral cortex Na+/K+ ATPase at 1 mM by colorimetric microplate-based assay
|
Sus scrofa
|
100.0
%
|
|
|
PUBCHEM_BIOASSAY: Luminescence Cell-Based HTS Dose Confirmation to Identify Inhibitors of of 5'UTR Stem-Loop Driven Alpha-Synuclein mRNA Translation in H4 Neuroglioblastoma Cells. (Class of assay: confirmatory) [Related pubchem assays: 1813 (Primary HTS), 1827 (Project Summary)]
|
None
|
64.0
nM
|
|
|
PUBCHEM_BIOASSAY: Luminescence Cell-Based Dose Response HTS to Identify Inhibitors of Luciferase Translation or Activity in H4 Neuroglioblastoma Cells. (Class of assay: confirmatory) [Related pubchem assays: 1813 (Primary HTS), 1827 (Project Summary)]
|
None
|
100.0
nM
|
|
|
PUBCHEM_BIOASSAY: Luminescence Cell-Based Dose Response to Identify Inhibitors of Luciferase Translation or Activity in H4-C Neuroglioblastoma Cells. (Class of assay: confirmatory) [Related pubchem assays: 1813 (Primary HTS), 1827 (Project Summary)]
|
None
|
84.0
nM
|
|
|
PUBCHEM_BIOASSAY: Luminescence-based dose response cell-based high throughput screening assay for inhibitors of kruppel-like factor 5 (KLF5). (Class of assay: confirmatory) [Related pubchem assays: 1700 (Primary screen.), 1834 (Confirmation screen.), 1905 (Counterscreen.), 1858 (Summary AID.)]
|
Homo sapiens
|
164.55
nM
|
|
|
PUBCHEM_BIOASSAY: Luminescence Cell-Based Dose Response to Identify Inhibitors of 5'UTR Stem-Loop Driven Prion Protein mRNA Translation in H4-C Neuroglioblastoma Cells. (Class of assay: confirmatory) [Related pubchem assays: 1827 (Project Summary), 1813 (Primary HTS)]
|
None
|
80.0
nM
|
|
|
PUBCHEM_BIOASSAY: Luminescence Cell-Based Dose Response HTS to Identify Inhibitors of 5'UTR Stem-Loop Driven Prion Protein mRNA Translation in H4 Neuroglioblastoma Cells. (Class of assay: confirmatory) [Related pubchem assays: 1827 (Project Summary), 1813 (Primary HTS)]
|
None
|
100.0
nM
|
|
|
PUBCHEM_BIOASSAY: Luminescence Cell-Based Dose Confirmation HTS to Identify Inhibitors of of 5'UTR Stem-Loop Driven Alpha-Synuclein mRNA Translation in H4 Neuroglioblastoma Cells. (Class of assay: confirmatory) [Related pubchem assays: 1827 (Project Summary), 1813 (Primary HTS)]
|
None
|
100.0
nM
|
|
|
PUBCHEM_BIOASSAY: Late stage assay provider counterscreen results from the probe development effort to identify inhibitors of kruppel-like factor 5 (KLF5): chemiluminescence-based Western blot assay for inhibitors of KLF5 protein levels. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID1700, AID1825, AID1834, AID1858, AID1973, AID1975, AID2749, AID2750]
|
Homo sapiens
|
29.73
nM
|
|
|
PUBCHEM_BIOASSAY: Late stage assay provider counterscreen results from the probe development effort to identify inhibitors of kruppel-like factor 5 (KLF5): luminescence-based cell-based dose response assay for cytotoxic compounds using the DLD-1 cell line (Round 1). (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID1700, AID1825, AID1834, AID1858, AID1973, AID1975, AID2749, AID2750]
|
Homo sapiens
|
46.4
nM
|
|
|
PUBCHEM_BIOASSAY: A Cell-Based Confirmatory Screen for Compounds that Inhibit VEEV, TC-83. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID588723]
|
Chlorocebus sabaeus
|
50.0
nM
|
|
|
DRUGMATRIX: ATPase, Na+/K+ enzyme inhibition (substrate: ATP)
|
Sus scrofa
|
678.0
nM
|
|
|
Cytotoxicity against human T47D cells after 72 hrs by CellTiter-Glo assay
|
Homo sapiens
|
385.0
nM
|
|
|
Cytotoxicity against human SK-BR-3 cells after 72 hrs by CellTiter-Glo assay
|
Homo sapiens
|
403.0
nM
|
|
|
Cytotoxicity against human Hs578T cells after 72 hrs by CellTiter-Glo assay
|
Homo sapiens
|
110.0
nM
|
|
|
Cytotoxicity against human Hs578Bst cells after 72 hrs by CellTiter-Glo assay
|
Homo sapiens
|
6.0
nM
|
|
|
Inhibition of electric eel AChE at 2 mg/ml by Ellman's method
|
Electrophorus electricus
|
-3.72
%
|
|
|
Inhibition of horse BChE at 2 mg/ml by Ellman's method
|
Equus caballus
|
5.11
%
|
|
|
Inhibition of human liver OATP1B1 expressed in HEK293 Flp-In cells assessed as reduction in E17-betaG uptake at 20 uM by scintillation counting
|
Homo sapiens
|
50.5
%
|
|
|
Inhibition of human liver OATP1B3 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E17-betaG uptake at 20 uM incubated for 5 mins by scintillation counting
|
Homo sapiens
|
20.5
%
|
|
|
Inhibition of human liver OATP2B1 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E3S uptake at 20 uM incubated for 5 mins by scintillation counting
|
Homo sapiens
|
-24.1
%
|
|
|
Cytotoxicity against human SK-MEL-28 cells after 3 days by MTT assay
|
Homo sapiens
|
211.0
nM
|
|
|
Cytotoxicity against human U373 cells after 3 days by MTT assay
|
Homo sapiens
|
77.0
nM
|
|
|
Cytotoxicity against human A549 cells after 3 days by MTT assay
|
Homo sapiens
|
27.0
nM
|
|
|
Cytotoxicity against human PC3 cells after 3 days by MTT assay
|
Homo sapiens
|
43.0
nM
|
|
|
Cytotoxicity against human MCF7 cells after 3 days by MTT assay
|
Homo sapiens
|
133.0
nM
|
|
|
Cytotoxicity against human Hs683 cells after 3 days by MTT assay
|
Homo sapiens
|
28.0
nM
|
|
|
Inhibition of sodium fluorescein uptake in OATP1B1-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
|
Cricetulus griseus
|
108.07
%
|
|
|
Inhibition of sodium fluorescein uptake in OATP1B3-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
|
Cricetulus griseus
|
98.13
%
|
|
|
Inhibition of guinea pig kidney Na+-K+ dependent ATPase after 10 to 20 mins
|
Cavia porcellus
|
500.0
nM
|
|
|
Inhibition of calf heart Na+-K+ dependent ATPase after 10 to 20 mins
|
Bos taurus
|
500.0
nM
|
|
|
Inhibition of calf heart Na+-K+ dependent ATPase preincubated for 30 mins measured after 10 to 20 mins
|
Bos taurus
|
200.0
nM
|
|
|
Inhibition of rat brain sodium/potassium-transporting ATPase in presence of Mg2+ and Na+
|
Rattus norvegicus
|
500.0
nM
|
|
|
Inhibition of rat brain sodium/potassium-transporting ATPase after 10 mins in presence of Mg2+ and Na+
|
Rattus norvegicus
|
40.0
nM
|
|
|
Inhibition of Na+/K+ -ATPase in human intrauterine growth restriction placental membrane using 3-O-methylfluorescein phosphate by fluorometric method in presence of Kcl
|
Homo sapiens
|
3.4
ug.mL-1
|
|
|
Inhibition of recombinant rat Na+/K+-ATPase alpha2/beta1 expressed in baculovirus infected insect Sf9 cell membranes using [gamma-32P]ATP as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by liquid scintillation counting
|
Rattus norvegicus
|
170.0
nM
|
|
|
Inhibition of recombinant rat Na+/K+-ATPase alpha3/beta1 expressed in baculovirus infected insect Sf9 cell membranes using [gamma-32P]ATP as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by liquid scintillation counting
|
Rattus norvegicus
|
31.0
nM
|
|
|
Inhibition of recombinant rat Na+/K+-ATPase alpha4/beta1 expressed in baculovirus infected insect Sf9 cell membranes using [gamma-32P]ATP as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins in presence of Na+, K+ and Mg2+ by liquid scintillation counting
|
Rattus norvegicus
|
4.3
nM
|
|
|
Inhibition of recombinant rat Na+/K+-ATPase alpha4/beta3 expressed in baculovirus infected Sf9 cell membranes using [gamma-32P]ATP as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by liquid scintillation counting
|
Rattus norvegicus
|
1.8
nM
|
|
|
Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr
|
Chlorocebus sabaeus
|
97.0
nM
|
|
